International Journal of Biochemistry and Biophysics Vol. 8(1), pp. 1 - 8
DOI: 10.13189/ijbb.2020.080101
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Effect of Cnidoscolus aconitifolius Leaf Extract on Selected Renal Parameters and Hematological Indices of Carbon Tetrachloride Induced Toxic Rats

Iwu C. C. , Akwukwaegbu P. I. ^*, Ikewuchi C. C.
Department of Biochemistry, University of Port Harcourt, Nigeria

ABSTRACT

Effect of Cnidoscolus aconitifolius leaf extract on selected renal parameters and hematological indices of carbon tetrachloride (CCl₄) induced toxic rats was investigated using standard analytical methods. Forty-two (42) wistar rats weighing 91-185g was used for this study. The rats were divided into 7 groups of 6 rats each and allowed to acclimatize for one week with food and water ad libitum. Carbon tetrachloride was prepared in the ratio of 1:5 (v:v) in olive oil and administered subcutaneously at 0.17 mL/kg body weight on the first day after acclimatization. Group I received normal feed and water only, group II received CCl₄ only, group III received olive oil only, groups IV, V and VI received 50 mg/kgbw, 75 mg/kgbw and 100 mg/kgbw oral dose of C. aconitifolius respectively in addition to CCl₄ while group VII received vitamin C plus CCl₄. After oral administration for twenty-one days, the rats were painlessly sacrificed; plasma and blood were collected for renal and hematological analyses respectively. Results showed that CCl₄ increased Ca, Na, K and decreased RBC, PCV, Hb and platelet number of rats induced, however oral administration of C. aconitifolius significantly decreased; Ca and Na at 50 mg/kgbw, K in a dose-dependent pattern and significantly increased RBC, PCV, Hb and platelet number at all doses. This study has shown that the leaf extract of Cnidoscolus aconitifolius had a dose-dependent positive effect on the hematological indices as well as the potential to ameliorate kidney parameters of CCl₄ treated wistar rats at the given concentrations.

KEYWORDS
Cnidoscolus aconitifolius, Renal Parameters, Hematological Indices, Carbon Tetrachloride

Cite This Paper in IEEE or APA Citation Styles
(a). IEEE Format:
[1] Iwu C. C. , Akwukwaegbu P. I. , Ikewuchi C. C. , "Effect of Cnidoscolus aconitifolius Leaf Extract on Selected Renal Parameters and Hematological Indices of Carbon Tetrachloride Induced Toxic Rats," International Journal of Biochemistry and Biophysics, Vol. 8, No. 1, pp. 1 - 8, 2020. DOI: 10.13189/ijbb.2020.080101.

(b). APA Format:
Iwu C. C. , Akwukwaegbu P. I. , Ikewuchi C. C. (2020). Effect of Cnidoscolus aconitifolius Leaf Extract on Selected Renal Parameters and Hematological Indices of Carbon Tetrachloride Induced Toxic Rats. International Journal of Biochemistry and Biophysics, 8(1), 1 - 8. DOI: 10.13189/ijbb.2020.080101.