Universal Journal of Plant Science Vol. 1(4), pp. 118 - 124
DOI: 10.13189/ujps.2013.010402
Reprint (PDF) (220Kb)

Development of High-throughput Molecular Markers for Blackleg (Leptosphaeria maculans) Resistance Genes in Brassica napus for Gene Stacking

Zining Wang ^1,2,*
1 Department of Plant Science, University of Manitoba, Winnipeg MB R3T 2N2, Canada
² Current address: Plant Genome Mapping Laboratory, UGA, 111 Riverbend Road, Athens, GA 30602, USA

ABSTRACT

‘Quinta’ and ‘Glacier’ are two important winter type canola cultivars from Europe. ‘Quinta’ has two independent blackleg resistance gene loci, Rlm1 and Rlm4; ‘Glacier’ has two other genes, Rlm2 and Rlm3. This study focuses on mapping the resistance genes Rlm1 and Rlm3 using high throughput SRAP (sequence related amplified polymorphism) markers and SNPs. The F₂ and F₃ populations were constructed with crosses of ‘Westar’ × ‘Quinta’ and ‘Westar’ × ‘Glacier’. The populations were screened with the corresponding isolates. The phenotype segregation in the populations supported that a single dominant resistance gene controls the resistance in ‘Quinta’ and “Glacier” separately. The closest SRAP marker was identified for the resistance gene in ‘Quinta’ by using 256 pairs of primers and screening 2,500 polymorphic loci. B342 is 1.5 cM to Rlm1 and 6.8 cM to Rlm3. SNP80870 developed from the N7 orthologous region in Arabidopsis was 3 cM to Rlm3. A population for stacking the three resistance genes (Rlm1, Rlm3 and LepR3) was constructed. Marker screening and disease inoculation were both used for the selection of lines with the three resistance genes.

KEYWORDS
Blackleg, Resistance Gene, Marker, Mapping, Gene Stacking, B. Napus

Cite This Paper in IEEE or APA Citation Styles
(a). IEEE Format:
[1] Zining Wang , "Development of High-throughput Molecular Markers for Blackleg (Leptosphaeria maculans) Resistance Genes in Brassica napus for Gene Stacking," Universal Journal of Plant Science, Vol. 1, No. 4, pp. 118 - 124, 2013. DOI: 10.13189/ujps.2013.010402.

(b). APA Format:
Zining Wang (2013). Development of High-throughput Molecular Markers for Blackleg (Leptosphaeria maculans) Resistance Genes in Brassica napus for Gene Stacking. Universal Journal of Plant Science, 1(4), 118 - 124. DOI: 10.13189/ujps.2013.010402.