International Journal of Biochemistry and Biophysics Vol. 6(1), pp. 1 - 19
DOI: 10.13189/ijbb.2018.060101
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Fabrication and Characterization of Layered Graphene Oxide Biocompatible Nano-Film by Various Methods

Himanshu Kharkwal ^*, H.C. Joshi , K.P. Singh
Membrane Biophysics and Nanobiosensor Research Laboratory, College of Basic Sciences and Humanities, G.B. Pant University of Agriculture and Technology, Pantnagar Udham Singh Nagar-263145, India

ABSTRACT

The present study includes preparation, fabrication and characterization of GO, GO coated PCTE and Egg membrane respectively. Characterization of GO was done by UV-Visible spectrophotometery, Fourier Transform Infrared spectroscopy (FT-IR), Energy Dispersive Spectroscopy (EDS), and Scanning Electron Microscopy (SEM). Different membranes i.e. PCTE (Polycarbonate track etch membrane) and egg membrane are used for the coating of graphene oxide. The egg membrane was isolated from the egg shell by dipping the egg in 8% HCl solution. These membrane i.e. PCTE and egg membrane were then fabricated with graphene oxide by drop casting method and vacuum filtration. The coated membrane was then characterized by different imaging technique such as FE-SEM, Inverted microscopy, FT-IR, and EDX. Based on the characterization best suited method for coating was determined and we found vacuum filtration method as the best method for coating. The simple (non-coated) and GO coated membrane of Egg and PCTE were then used for checking the potential gradient across the membrane by using digital multimeter and from this we came to conclusion that the potential gradient across the GO coated PCTE membrane is highest and lowest potential is of GO coated Egg membrane. Above all we found that GO coated egg membrane is highly beneficial and cost effective as compared to artificial membranes.

KEYWORDS
Graphene Oxide (GO), Polycarbonate Track Etched (PCTE) Membrane, Fourier Transform Infrared Spectroscopy (FT-IR), Energy Dispersive Spectroscopy (EDS), and Scanning Electron Microscopy (SEM)

Cite This Paper in IEEE or APA Citation Styles
(a). IEEE Format:
[1] Himanshu Kharkwal , H.C. Joshi , K.P. Singh , "Fabrication and Characterization of Layered Graphene Oxide Biocompatible Nano-Film by Various Methods," International Journal of Biochemistry and Biophysics, Vol. 6, No. 1, pp. 1 - 19, 2018. DOI: 10.13189/ijbb.2018.060101.

(b). APA Format:
Himanshu Kharkwal , H.C. Joshi , K.P. Singh (2018). Fabrication and Characterization of Layered Graphene Oxide Biocompatible Nano-Film by Various Methods. International Journal of Biochemistry and Biophysics, 6(1), 1 - 19. DOI: 10.13189/ijbb.2018.060101.