Universal Journal of Agricultural Research Vol. 5(6), pp. 344 - 349
DOI: 10.13189/ujar.2017.050605
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Morphological and Genetical Study on Rhizoctonia Sheath Disease Complex of Rice in Bangladesh


Mohammod Hossain 1,*, Surapareddy Sreenivasaprasad 2, Muthu Meena 3, Nitya Ranjan Sharma 1
1 Plant Pathology Division, Bangladesh Rice Research Institute (BRRI), Gazipur, Bangladesh
2 School of Life Sciences, University of Bedfordshire, Bedfordshire, UK
3 Warwick Crop Centre, University of Warwick, Warwick, UK

ABSTRACT

Morphological and molecular based experiments were conducted to identify the presence of Rhizoctonia solani, R. oryzae and R. oryzae-sativae which were the causal agents of sheath blight and sheath blight-like symptoms associated with sheath disease complex of rice. Disease survey was conducted at Bogra, Comilla, Gazipur and Rajshahi Districts in Bangladesh. The data collected revealed that sheath blight was the most important disease (19.8%) followed by aggregated sheath spot (2.4%), and sheath spot (2.3%). Three pathogens of the disease complex were identified morphologically by their spot size, general appearance of spot, number of spot, position in plant, sclerotium visibility and sclerotial size on infected plant. Those three species of Rhizoctonia of rice sheath disease complex were confirmed by diagnostic-PCR with their corresponding reference isolates by using specific primers GMRS-3, GMRO-3, GMROS-2 to Rhizoctonia solani, R. oryzae and R. oryzae-sativae, respectively. Molecular approaches might overcome the limitations of morphological characterization particularly when huge samples were needed to distinguish.

KEYWORDS
Rice, Rhizoctonia Spp., Disease Complex, Diagnostic-PCR

Cite This Paper in IEEE or APA Citation Styles
(a). IEEE Format:
[1] Mohammod Hossain , Surapareddy Sreenivasaprasad , Muthu Meena , Nitya Ranjan Sharma , "Morphological and Genetical Study on Rhizoctonia Sheath Disease Complex of Rice in Bangladesh," Universal Journal of Agricultural Research, Vol. 5, No. 6, pp. 344 - 349, 2017. DOI: 10.13189/ujar.2017.050605.

(b). APA Format:
Mohammod Hossain , Surapareddy Sreenivasaprasad , Muthu Meena , Nitya Ranjan Sharma (2017). Morphological and Genetical Study on Rhizoctonia Sheath Disease Complex of Rice in Bangladesh. Universal Journal of Agricultural Research, 5(6), 344 - 349. DOI: 10.13189/ujar.2017.050605.