Universal Journal of Applied Science Vol. 5(4), pp. 27 - 33
DOI: 10.13189/ujas.2017.050401
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Application of Root Maceration of Common Weeds: T. diversifolia and S. jamaicensis for Phytoremediation of Ohaji Egbema Crude Oil Polluted Soils


Amadi, P.U 1,*, Agomuo, E.N 2, Akpobasaha N.A 1, Njoku, U.C 1
1 Department of Biochemistry, University of Port Harcourt, Choba Rivers State, Nigeria
2 Department of Biochemistry, Imo State University Owerri, Imo State, Nigeria

ABSTRACT

This study examined the remediation of crude oil polluted soils obtained from Ohaji Egbema Imo State Nigeria using macerated roots of T. diversifolia (TD) and S. jamaicensis (SJ). The soil pH levels, PAHs (Polycyclic aromatic hydrocarbons) and TPH (Total petroleum hydrocarbons) contents, and catalase and peroxidase activities were evaluated. Thirty-two polyethene bags containing 300g of soil samples were divided into eight groups of quadruplicates. Group 1 were the unpolluted soil samples (USS), group 2; polluted soil samples (PSS) without remediation, group 3; PSS containing 250g of TD, group 4; PSS containing 500g of TD, group 5; PSS containing 250 g of SJ, group 6; PSS containing 500g of SJ, group 7; PSS containing 250g of the mixture of TD and SJ (1:1) and group 8; PSS containing 500g of the mixture of TD and SJ (1:1). At all experimental periods, the pH of the PSS without remediation was significantly lower than the USS. The 500 g TD completely normalized the altered pH of the PSS on the 4th week, comparable to the results of the 12th week. The PAH (Polycyclic aromatic hydrocarbons) levels in the group 5 and 7 on the 1st day were not significantly different from the PAH of the group 2. Group 3, 4, 7, and 8 produced the best PAH lowering effects on the polluted soils on the 8th week, which compared to the results of the 12th week. At all experimental periods, 500g of TD provided the most effective for TPH reduction of the polluted soils. Results for the enzyme activities showed a significantly decreased (p < 0.05) catalase and peroxidase levels of the polluted soils when compared to the USS. Both the 1st day and 1st week recorded similar catalase activities in all groups except group 5, while group 4-8 recorded significantly increased catalase activities from the 4 - 12th week. For the 1st day, 1st week, and 4th week, increasing the amount of the plants from 250g - 500g had no effect on the peroxidase levels of the soils, while a quantity dependent significant increase in peroxidase levels was observed only for group 7 and 8 on the 8th and 12th weeks. The 500g TD produced the most significant restorative effect on the polluted soils. This study has shown the extensive remediation produced by TD on crude oil polluted soils of Ohaji Egbema.

KEYWORDS
T. diversifolia, S. jamaicensis, Remediation, Enzymes, Hydrocarbons

Cite This Paper in IEEE or APA Citation Styles
(a). IEEE Format:
[1] Amadi, P.U , Agomuo, E.N , Akpobasaha N.A , Njoku, U.C , "Application of Root Maceration of Common Weeds: T. diversifolia and S. jamaicensis for Phytoremediation of Ohaji Egbema Crude Oil Polluted Soils," Universal Journal of Applied Science, Vol. 5, No. 4, pp. 27 - 33, 2017. DOI: 10.13189/ujas.2017.050401.

(b). APA Format:
Amadi, P.U , Agomuo, E.N , Akpobasaha N.A , Njoku, U.C (2017). Application of Root Maceration of Common Weeds: T. diversifolia and S. jamaicensis for Phytoremediation of Ohaji Egbema Crude Oil Polluted Soils. Universal Journal of Applied Science, 5(4), 27 - 33. DOI: 10.13189/ujas.2017.050401.