International Journal of Biochemistry and Biophysics Vol. 5(3), pp. 53 - 64
DOI: 10.13189/ijbb.2017.050301
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Application of Nanopore of Solid Membrane for Recognition of fluorescent Pseudomonas

H. C. Joshi ^1,*, K. P. Singh ¹, Abhishek Tomar ², Prashant Singh ^3
1 Membrane Biophysics and Nanobiosensor Research Laboratory, College of Basic Sciences and Humanities, G. B. Pant University of Agriculture and Technology, India
² Department of Electronics & Communication Engineering, G. B. Pant University of Agriculture & Technology, Pantnagar, India
³ Department of Biotechnology, Bhimtal Campus, Kumaun University, India

ABSTRACT

The uses of biosensors for sensitive and specific detection of various analytes are of great importance, and its success is often dictated by the nature of the detection element (the specific ligand) and the choice of target analytes. The conventional agriculture caused considerable impacts on soils and waters. It is important to change certain agricultural managements to environmental cleaner techniques. The sustainable agriculture has pointed many approaches and techniques to reduce environmental impact. One of those strategies is the utilization of the soil microbiota to induce plant growth, control of plant diseases and biodegradation of xenobiotic compounds. Studies on the relationship between roots and microbiota are essential to achieve agricultural applications. These studies indicate that one of the most abundant microorganisms in the rhizosphere (area around the roots) is fluorescent Pseudomonas spp. They have been considered as an alternative to agrochemicals for controlling plant diseases and increasing plant development. In the present study gold coated polycarbonate membrane of pore size 100 nm was used. The coated membrane carries carboxylic, thiol and hydroxyl groups. Carboxylic groups are being activated by treating the coated nanoporous membrane with the NHS-EDC linker for the binding of antibodies against the pathogen (fluorescent Pseudomonas) to be detected. After the activation of the membrane, antibodies (which are generated against the fluorescent Pseudomonas) were immobilized over the membrane in the humid chamber. After leaving it for an hour, unbound antibodies were washed by Phosphate buffer Saline. The remaining sites on membrane were then blocked by the Bovine serum albumin. The membrane was then subjected for the detection of pathogen by the electrochemical immunosensor by observing the change in the impedance value. The characterization of such membrane was done by Fourier Transform Infrared Spectroscopy (FT-IR), Scanning Electron Microscopy (SEM), Energy Dispersive Spectroscopy (EDS) and Thermo gravimetric Analyzer (TGA).

KEYWORDS
Antibody-Antigen (Ab-Ag), Gold (Au), Nanoporous Polycarbonate Track Etched (PCTE) Membrane, Fourier Transform Infrared Spectroscopy (FT-IR), Scanning Electron Microscopy (SEM), Energy Dispersive Spectroscopy (EDX/EDS)

Cite This Paper in IEEE or APA Citation Styles
(a). IEEE Format:
[1] H. C. Joshi , K. P. Singh , Abhishek Tomar , Prashant Singh , "Application of Nanopore of Solid Membrane for Recognition of fluorescent Pseudomonas," International Journal of Biochemistry and Biophysics, Vol. 5, No. 3, pp. 53 - 64, 2017. DOI: 10.13189/ijbb.2017.050301.

(b). APA Format:
H. C. Joshi , K. P. Singh , Abhishek Tomar , Prashant Singh (2017). Application of Nanopore of Solid Membrane for Recognition of fluorescent Pseudomonas. International Journal of Biochemistry and Biophysics, 5(3), 53 - 64. DOI: 10.13189/ijbb.2017.050301.