International Journal of Biochemistry and Biophysics Vol. 5(1), pp. 26 - 36
DOI: 10.13189/ijbb.2017.050104
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Xenon-Water Interaction in Bacterial Suspensions as Studied by NMR

Victor Rodin ^1,*, Alexander Ponomarev ², Maxim Gerasimov ³, Leonid Gurevich ^4
1 Institute of Organic Chemistry, Johannes Kepler University Linz, Austria
² Department of Medical Cell Technologies, Ural State Medical University, Russian Federation
³ Department of Clinical and Experimental Medicine, Linköping University, Sweden
⁴ Department of Physics and Nanotechnology, Aalborg University, Denmark

ABSTRACT

Xenon is a perspective gas for creation of oxygen free environment for different applications of biomaterials. To use xenon in suspensions and products properly it is necessary to know the molecular mechanisms of its interactions with water and cells. This work reports the study of bacterial suspensions of Escherichia coli in the presence of xenon using nuclear magnetic resonance (NMR). The work studied how the spin-lattice relaxation times of water protons in suspension change under xenon conditions. Xenon is able to form clathrate hydrates with water molecules at a temperature above the melting point of ice. The work studied NMR relaxation times which reflect the rotation freedom of water molecules in suspension. Lower relaxation times indicate reduced rotational freedom of water. Single exponential behavior of spin-lattice relaxation of protons in the suspensions of microorganisms has been registered. A recovery of longitudinal magnetization in cell suspensions with xenon clathrates has been characterized by two peaks in T₁-distribution. Fast relaxing T₁-component was related to the intracellular water and depended on the amount of xenon clathrates. The obtained results elucidate how the NMR method can monitor the process of clathrate formation and how the xenon atoms and hydrates interact with cells.

KEYWORDS
Bacterial Suspensions, Water, Xenon, Clathrates, Nuclear Magnetic Resonance

Cite This Paper in IEEE or APA Citation Styles
(a). IEEE Format:
[1] Victor Rodin , Alexander Ponomarev , Maxim Gerasimov , Leonid Gurevich , "Xenon-Water Interaction in Bacterial Suspensions as Studied by NMR," International Journal of Biochemistry and Biophysics, Vol. 5, No. 1, pp. 26 - 36, 2017. DOI: 10.13189/ijbb.2017.050104.

(b). APA Format:
Victor Rodin , Alexander Ponomarev , Maxim Gerasimov , Leonid Gurevich (2017). Xenon-Water Interaction in Bacterial Suspensions as Studied by NMR. International Journal of Biochemistry and Biophysics, 5(1), 26 - 36. DOI: 10.13189/ijbb.2017.050104.