International Journal of Biochemistry and Biophysics Vol. 4(3), pp. 31 - 36
DOI: 10.13189/ijbb.2016.040302
Reprint (PDF) (326Kb)

Evaluation of Antioxidant Activities and Estimation of Zinc Content of Aqueous and Methanolic Extracts of Three Medicinal Plants: Cochlospermum Planchonii, Pericopsis Laxiflora and Harungana Madagascariensis

Koffi A. Jeanne ^1,*, Bla K. Brice ¹, Silué K. Dieudonné ², Tuo Karim ³, Yapi H. Félix ^1
1 Laboratory of Biochemical-Pharmacodynamics, Felix Houphouet Boigny University, Cote d'Ivoire
² Environment and Health Department, Swiss Centre for Scientific Research, Cote d'Ivoire
³ Department of Parasitology, Institut Pasteur de Cote d'Ivoire, Cote d'Ivoire

ABSTRACT

Malaria disease is a pathology which occurs as a result of the oxidative stress. Three plants of the Ivorian pharmacopoeia are particularly used in the traditional medicine for the treatment of malaria in Côte d'Ivoire. They are Cochlospermum planchonii (Placo), Pericopsis laxiflora (Laper) and Harungana madagascariensis (Madhar).The objective of this study was to evaluate the in vitro antioxidant activity of the aqueous and methanolic extracts of three medicinal plants of Côte d'Ivoire. The quantitative measurement of total flavonoids gave the following results: Laper (560.80 mg QE/g ± 7.40), Madhar (352.50 mg QE/g ± 9.46) and Placo (314.20 mg QE/g ± 4.41). The total polyphenols were more concentrated in Laper (262.50 mg GAE/g ± 9.47) and Placo (203.20 mg GAE/g ± 2.21). Noticeable zinc content was found in Laper (13.56 ppm) and Placo (11.40 ppm) against 3.43 ppm in Madhar. The radical scavenging activity by 2,2-diphenyl-1-picryl hydrazyl (DPPH) and antiradical power measurement by ABTS (2,2' azino bis-3-ethylbenzothiazoline-6-sulphonic acid) assay have showed good activity for Laper (IC₅₀ = 7.50 μg/mL) and placo (IC₅₀ = 8.01 μg/mL). The standard antioxidant compound (quercetin) gave an IC₅₀ = 4.00 μg/mL. This study suggested that the high content of total flavonoids, total phenols and minerals in these plants could partly justify the therapeutic use of at least two of the three plants.

KEYWORDS
Antioxidant Activity, Polyphenols, Zinc, Medicinal Plants, Côte d'Ivoire

Cite This Paper in IEEE or APA Citation Styles
(a). IEEE Format:
[1] Koffi A. Jeanne , Bla K. Brice , Silué K. Dieudonné , Tuo Karim , Yapi H. Félix , "Evaluation of Antioxidant Activities and Estimation of Zinc Content of Aqueous and Methanolic Extracts of Three Medicinal Plants: Cochlospermum Planchonii, Pericopsis Laxiflora and Harungana Madagascariensis," International Journal of Biochemistry and Biophysics, Vol. 4, No. 3, pp. 31 - 36, 2016. DOI: 10.13189/ijbb.2016.040302.

(b). APA Format:
Koffi A. Jeanne , Bla K. Brice , Silué K. Dieudonné , Tuo Karim , Yapi H. Félix (2016). Evaluation of Antioxidant Activities and Estimation of Zinc Content of Aqueous and Methanolic Extracts of Three Medicinal Plants: Cochlospermum Planchonii, Pericopsis Laxiflora and Harungana Madagascariensis. International Journal of Biochemistry and Biophysics, 4(3), 31 - 36. DOI: 10.13189/ijbb.2016.040302.