International Journal of Biochemistry and Biophysics Vol. 4(3), pp. 25 - 30
DOI: 10.13189/ijbb.2016.040301
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Evaluation of Antioxidant Activity of Aqueous and Ethanolic Extracts of Stem Bark of Xylopia villosa Chipp (Annonaceae)

Kouame Yao Yves ^1,*, Okpekon Aboua Timothée ², Niamketchi Gilles Léonce ³, Gbassi Komenan Gildas ⁴, Yapi Houphouët Félix ^1
1 Laboratory of Pharmacodynamics Biochemical, UFR Biosciences, Felix Houphouet Boigny University, Cote d’Ivoire
² Laboratory of Organic Chemistry and Natural Substances, UFR Sciences of Structures of Matter and Technology, Felix Houphouet Boigny University, Cote d’Ivoire
³ Biochemistry Laboratory and Food Science, Felix Houphouet Boigny University, Cote d’Ivoire
⁴ Laboratory of Analytical Chemistry, General and Inorganic Chemistry, Faculty of Science Pharmaceutical and Biological, Felix Houphouet Boigny University, Cote d’Ivoire

ABSTRACT

This study aims to evaluate antioxidant activity of aqueous and ethanolic extracts of Xylopia villosa stem bark. The quantitative study of polyphenols showed that ethanolic extract (75.00 ± 3.82 g/L EqAG) contains more than the aqueous extract (55.83 ± 1.67 g/L EqAG). It is the same for the quantitative analysis of total flavonoids where ethanolic extract (135.00 ± 1.44 g/L EqQ) contains more than the aqueous extract (115.00 ± 6.61 g/L EqQ). The antioxidant activity in vitro showed that the extracts neutralize DPPH and ABTS + ^° with enhanced activity of the ethanolic extract. The ethanolic and aqueous extracts of Xylopia villosa causing 50% inhibition (IC50) of the DPPH radical gave concentrations of 10.50 ± 0.24 and 24.50 ± 0.18 mcg / ml respectively for ethanol extract and aqueous extract. On the reduction of radical ABTS + ^°, aqueous extract caused a reduction of the radical cation ABTS + 7.82 ± 0.17 micromol TE / L while the ethanol extract reduced by 10.52 ± 0.27 micromol TE / L. Evaluation of antioxidant activity in vivo of xylopia villosa revealed that this is the ethanol extract (9.17 ± 0.48 mmol / L TBA-MDA) which offset the concentration of TBA-MDA adduct more than the aqueous extract (22.67 ± 0.33 mmol / L TBA-MDA). Concerning the total antioxidant activity, ethanolic extract reduced iron III to Iron II more than aqueous extract. After this study, it appears that the ethanolic extract has better antioxidant activity in vitro and in vivo that the aqueous extract.

KEYWORDS
Xylopia villosa, Antioxidant, In vitro, Carrageenan

Cite This Paper in IEEE or APA Citation Styles
(a). IEEE Format:
[1] Kouame Yao Yves , Okpekon Aboua Timothée , Niamketchi Gilles Léonce , Gbassi Komenan Gildas , Yapi Houphouët Félix , "Evaluation of Antioxidant Activity of Aqueous and Ethanolic Extracts of Stem Bark of Xylopia villosa Chipp (Annonaceae)," International Journal of Biochemistry and Biophysics, Vol. 4, No. 3, pp. 25 - 30, 2016. DOI: 10.13189/ijbb.2016.040301.

(b). APA Format:
Kouame Yao Yves , Okpekon Aboua Timothée , Niamketchi Gilles Léonce , Gbassi Komenan Gildas , Yapi Houphouët Félix (2016). Evaluation of Antioxidant Activity of Aqueous and Ethanolic Extracts of Stem Bark of Xylopia villosa Chipp (Annonaceae). International Journal of Biochemistry and Biophysics, 4(3), 25 - 30. DOI: 10.13189/ijbb.2016.040301.