Universal Journal of Applied Science Vol. 1(2), pp. 33 - 37
DOI: 10.13189/ujas.2013.010201
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DNA Insecticides: Application of the Iap-2 Gene Single-stranded Fragments from Three Different Nucleopolyhedroviruses against Second Instar Gypsy Moth Larvae


Volodymyr V. Oberemok1,*, Andrey P. Simchuk1, Yuri I. Gninenko2
1 Department of Biochemistry, Taurida National V.I. Vernadsky University, 95007 Simferopol, Ukraine
2 All-Russian Research Institute of Silviculture and Mechanization of Forestry, 141200 Pushkino, Russia

ABSTRACT

We discovered that the external application of a solution containing one single-stranded DNA fragment, 18 nucleotides long, either from the BIR domain of the AcMNPV IAP-2 gene or the RING domain of the TnSNPV IAP-2 gene, induces a significantly higher mortality of gypsy moth larvae compared to a solution of distilled water. The results show the insecticidal potential of viral DNA fragments that can be used to create safe, relatively inexpensive and fast-acting insecticides to control quantity of pest insect populations. In our opinion, insecticidal effect may depend on the specific sequence of double and triple hydrogen bonds in a single-stranded DNA fragment or on a sequence of a few nitrogenous bases at specific sites of an oligonucleotide. The results suggest that small single-stranded viral DNA fragments may have practical applications as DNA insecticides.

KEYWORDS
DNA Insecticides, Anti-Apoptotic Gene IAP-2, Gypsy Moth, Nucleopolyhedrovirus, Pest Control

Cite This Paper in IEEE or APA Citation Styles
(a). IEEE Format:
[1] Volodymyr V. Oberemok , Andrey P. Simchuk , Yuri I. Gninenko , "DNA Insecticides: Application of the Iap-2 Gene Single-stranded Fragments from Three Different Nucleopolyhedroviruses against Second Instar Gypsy Moth Larvae," Universal Journal of Applied Science, Vol. 1, No. 2, pp. 33 - 37, 2013. DOI: 10.13189/ujas.2013.010201.

(b). APA Format:
Volodymyr V. Oberemok , Andrey P. Simchuk , Yuri I. Gninenko (2013). DNA Insecticides: Application of the Iap-2 Gene Single-stranded Fragments from Three Different Nucleopolyhedroviruses against Second Instar Gypsy Moth Larvae. Universal Journal of Applied Science, 1(2), 33 - 37. DOI: 10.13189/ujas.2013.010201.