Bioengineering and Bioscience(CEASE PUBLICATION) Vol. 4(2), pp. 20 - 23
DOI: 10.13189/bb.2016.040202
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Estimation of Sulphur Containing Amino Acids in Soybean Products in Nigeria

David Ebuka Elijah ^1,*, Nwobodo Amarachukwu Grace ², Okafor Chinedu Arthur ^3
1 Department of Chemistry, Biochemistry/Molecular Biology, Federal University Ndufu Alike, Nigeria
² Department of Biochemistry, Renaissance University, Nigeria
³ Department of Microbiology, Renaissance University, Nigeria

ABSTRACT

Five different samples of soybean products (Raw Soybeans Seed (RSS), Local Soybean Powdered Milk (LSPM), Local Soybean Liquid Milk (LSLM), Vita-milk and Chi-soymilk) were used for the analysis of sulphur containing amino acids (methionine and cysteine) contents. Qualitative analysis using thin layer chromatography technique was carried out on the soybean products to detect the presence of sulphur containing amino acids while spectrophotometric method involving methionine and cysteine standards were used to quantitatively estimate the two amino acids in the soybean products. The RF values (Retardation Factor) of 0.70 and 0.90 for methionine and cysteine standards respectively were used as control values. The presence of methionine in these five soybean products was detected with RF values of 0.69, 0.70, 0.70, 0.70 and 0.71 for RSS, LSPM, LSLM, Vita-milk and Chi-soymilk, respectively. The presence of cysteine in these five samples of soybean products was detected with RF values 0.91, 0.92, 0.90, 0.90 and 0.92 for the five samples. The result of qualitative analysis confirms the presence of methionine and cysteine in all the samples. From the result of the spectrophometric analysis of the products, methionine concentration in (g/100ml) present in Vita-milk (0.90±0.70) and Chi-soymilk (0.64±0.02) is significantly higher (p<0.05) than in the control RSS (0.47±0.02). Methionine concentration (g/100ml) present in Local Soybean Powdered Milk (LSPM), (0.22±0.02) is significantly lower than RSS (0.47±0.02) but not for Local Soybean Liquid Milk (LSLM) (0.50±0.02). Cysteine concentration (g/100ml) present in Vita-milk (1.130±0.27), Chi-soymilk (0.590±0.06) and Local Soybean Liquid Milk (0.610±0.03) is significantly higher (p<0.05) than the RSS control (0.130±0.03). Also cysteine concentration (g/100ml) present in Local Soybean Powdered Milk (0.126±0.02) is significantly lower (p<0.05) than the RSS control (0.130±0.03). An increase in both methionine and cysteine concentrations for Chi-soymilk and Vita-milk suggests that these soybean products were enriched with these amino acids during processing, hence can effectively replenish the lost sulphur amino acids in the body due to the action of trypsin inhibitor present in soybeans.

KEYWORDS
Soybean Products, Amino Acids, Sulphur Amino Acids

Cite This Paper in IEEE or APA Citation Styles
(a). IEEE Format:
[1] David Ebuka Elijah , Nwobodo Amarachukwu Grace , Okafor Chinedu Arthur , "Estimation of Sulphur Containing Amino Acids in Soybean Products in Nigeria," Bioengineering and Bioscience(CEASE PUBLICATION), Vol. 4, No. 2, pp. 20 - 23, 2016. DOI: 10.13189/bb.2016.040202.

(b). APA Format:
David Ebuka Elijah , Nwobodo Amarachukwu Grace , Okafor Chinedu Arthur (2016). Estimation of Sulphur Containing Amino Acids in Soybean Products in Nigeria. Bioengineering and Bioscience(CEASE PUBLICATION), 4(2), 20 - 23. DOI: 10.13189/bb.2016.040202.