Universal Journal of Agricultural Research Vol. 3(6), pp. 211 - 219
DOI: 10.13189/ujar.2015.030605
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Research Progress on Reference Genes of Insect for Quantitative Real-time Reverse Transcription PCR (RT-qPCR)


Cai-hua Shi 1,*, Jing-rong Hu 1, You-jun Zhang 1,2
1 College of Agriculture, Yangtze University, China
2 Department of Plant Protection, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Science, China

ABSTRACT

Quantitative real-time reverse transcription PCR (RT-qPCR) has become the most important method for the quantification of mRNA transcription levels owing to its, specificity, sensitivity, reproducibility, and efficiency. In order to avoid sample-to-sample and run-to-run variations particularly in RNA extraction, RNA quality and cDNA reverse transcription level ,etc. it is necessary to use housekeeping gene which stably expressing as reference gene. Ideally, the housekeeping gene should not be regulated or influenced by the experimental procedure or co-regulated with the target gene. Studies insect models have shown that the expression levels of commonly used reference genes can differ among different tissue, organ types or physiological conditions. However, improper selection of reference genes will result in inaccurate calculation results and consequently obscure actual biological differences among samples, even opposite conclusion. Therefore, reference genes which specific stably expression in each experimental system should be selected in different insects and different experiments. This review aims to provide research achievements of domestic and foreign scholars on insect reference genes, which provide great promise for the future.

KEYWORDS
Reference Gene, Insect, RT-qPCR, Progress

Cite This Paper in IEEE or APA Citation Styles
(a). IEEE Format:
[1] Cai-hua Shi , Jing-rong Hu , You-jun Zhang , "Research Progress on Reference Genes of Insect for Quantitative Real-time Reverse Transcription PCR (RT-qPCR)," Universal Journal of Agricultural Research, Vol. 3, No. 6, pp. 211 - 219, 2015. DOI: 10.13189/ujar.2015.030605.

(b). APA Format:
Cai-hua Shi , Jing-rong Hu , You-jun Zhang (2015). Research Progress on Reference Genes of Insect for Quantitative Real-time Reverse Transcription PCR (RT-qPCR). Universal Journal of Agricultural Research, 3(6), 211 - 219. DOI: 10.13189/ujar.2015.030605.