Advances in Zoology and Botany Vol. 1(2), pp. 34 - 38
DOI: 10.13189/azb.2013.010203
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Subcellular Localization and Quantification of Camptothecin in Different Plant Parts of Chonemorpha Fragrans


Pradnya Kedari, Nutan Malpathak*
Department of Botany, University of Pune, Pune 411007, Maharashtra, India

ABSTRACT

Chonemorpha fragrans (C. fragrans), a liana belonging to family Apocynaceae, shows presence of widely used anticancer compound Camptothecin (CPT). CPT is a modified monoterpene indole alkaloid produced by very few species belonging to unrelated orders of angiosperms, especially plants belonging to the families Apocynaceae and Icacenaceae. In this work a sensitive high performance thin layer chromatography method (HPTLC) was used for detection and quantification of CPT. HPTLC analysis showed that highest amount of CPT was present in root methanolic extract (0.023% of dry weight) followed by stem bark methanolic extract (0.013% of dry weight). Also, the subcellular localization of an anticancer drug has been studied using autofluroscence property of CPT under Ultra violet (UV) light. Localization study revealed that out of all the organs studied roots accumulated highest levels of CPT followed by stem bark. Xylem tissues showed a major accumulation site for CPT. Presence of CPT in vitro culture is an important finding as in vitro systems can be used for further experiments to reduce overexploitation of natural population. This is the first report rearding subcellular localization and quantification of CPT in sequential extracts of different plant parts in C. fragrans.

KEYWORDS
Chonemorpha Fragrans, Apocynaceae, Anticancer, Camptothecin, HPLC, HPTLC, Localization

Cite This Paper in IEEE or APA Citation Styles
(a). IEEE Format:
[1] Pradnya Kedari , Nutan Malpathak , "Subcellular Localization and Quantification of Camptothecin in Different Plant Parts of Chonemorpha Fragrans," Advances in Zoology and Botany, Vol. 1, No. 2, pp. 34 - 38, 2013. DOI: 10.13189/azb.2013.010203.

(b). APA Format:
Pradnya Kedari , Nutan Malpathak (2013). Subcellular Localization and Quantification of Camptothecin in Different Plant Parts of Chonemorpha Fragrans. Advances in Zoology and Botany, 1(2), 34 - 38. DOI: 10.13189/azb.2013.010203.