Universal Journal of Plant Science Vol. 3(3), pp. 49 - 57
DOI: 10.13189/ujps.2015.030302
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Influence of Plant Metabolites on Flea Beetle Infestation in Spider Plant Morphotypes


Gatahi Dennis Maina 1, Wanyika Harrison 2, Gohole Linnet 3, Evans Ligare Chimoita 4,*
1 Department of Horticulture, Jomo Kenyatta University of Agriculture and Technology, Nairobi
2 Department of Chemistry, Jomo Kenyatta University of Agriculture and Technology, Nairobi
3 Department of crop Science, University of Eldoret, Eldoret
4 Department of Agricultural Economics, University of Nairobi, Kenya

ABSTRACT

Flea beetle is an important pest accounting for 25 % foliage damage in spider plant. The reported damage manifested as 'shot holes' is variable among the spider plant morphotypes. The study aimed at determining the damage inflicted on three main morphotypes in relation to metabolites. Three spider plant morphotypes were propagated inside a screen house on a completely randomised design (CRD) and flea beetles introduced on the seventh week of growth to determine the damage caused and assessed using the Bailey scoring scale (0-3, 4-7 and > 7 holes/leaf). Data on damage was log transformed and analysed using analysis of variance (ANOVA). The means were separated using protected least significant difference (LSD0.05). Quantitative determination of primary and secondary metabolites was done on the foliage taken before infestation. Proximate and Kjedahl methods were used to determine carbohydrates and proteins respectively. Determination of the secondary metabolites and vitamins was done using chromatography. The purple stemmed morphotype was most damaged (8.6, p≤0.05), green stemmed morphotype least damaged (2.7, p≤0.05), while the purple-green morphotype was moderately damaged (7.6, p≤0.05). There was a significant difference of metabolites among the morphotypes. Purple stemmed had highest carbohydrates, proteins, vitamins and glucosinolates but least terpenoids while green stemmed morphotype had the least primary metabolites and glucosinolates but highest terpenoids. The purple stemmed morphotype was most susceptible and green stemmed morphotype was least susceptible. The inflicted damage was largely metabolomically influenced and that primary metabolites and glucosinolates levels were antagonistic to the terpenoids levels in the morphotypes. It can be recommended that, plant breeders use the green stemmed morphotype to improve resistance of the more nutritious but flea beetle susceptible purple stemmed morphotype in order to increase yield and maintain the nutritional potential. Also farmers can grow green stemmed morphotype in flea beetle infested fields.

KEYWORDS
Flea Beetle, Spider Plant, Morphotype, Plant Metabolites, Damage

Cite This Paper in IEEE or APA Citation Styles
(a). IEEE Format:
[1] Gatahi Dennis Maina , Wanyika Harrison , Gohole Linnet , Evans Ligare Chimoita , "Influence of Plant Metabolites on Flea Beetle Infestation in Spider Plant Morphotypes," Universal Journal of Plant Science, Vol. 3, No. 3, pp. 49 - 57, 2015. DOI: 10.13189/ujps.2015.030302.

(b). APA Format:
Gatahi Dennis Maina , Wanyika Harrison , Gohole Linnet , Evans Ligare Chimoita (2015). Influence of Plant Metabolites on Flea Beetle Infestation in Spider Plant Morphotypes. Universal Journal of Plant Science, 3(3), 49 - 57. DOI: 10.13189/ujps.2015.030302.