Advances in Zoology and Botany Vol. 3(2), pp. 24 - 30
DOI: 10.13189/azb.2015.030203
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Effect of Carbon and Nitrogen Sources on the Growth, Reproduction and Ligninolytic Enzymes Activity of Dictyoarthrinium Synnematicum Somrith


Prasher IB *, Chauhan R
Mycology & Plant Pathology Laboratory, Department of Botany, Panjab University, India

ABSTRACT

The effect of different basal media, temperature, pH, days of incubation, carbon and nitrogen sources was studied on growth, reproduction and ligninolytic enzymes production of Dictyoarthrinium synnematicum Somrith. The fungus reproduced by chlamydospores and produced maximum growth in Glucose-peptone medium. It produced chlamydospores only at 24℃ and pH range of 5-9. The optimum mycelial growth rate was observed at 24℃ and pH 5 after 16 days of incubation. It showed gradual increase in the production of Lignin peroxidase up to 30 days, Manganese peroxidase up to 18 days of incubation after which it decreased and Laccase up to 22 days. The optimum growth and laccase activity were attained in lactose supplemented medium whereas the highest Lignin peroxidase and Manganese peroxidase activity was observed with glucose. It produced chlamydospores with fructose, glucose, maltose, sucrose and xylose. Sodium nitrate is the best inorganic nitrogen source for growth. Resting spores were formed in inorganic and organic nitrogen sources used in basal medium. Ammonium phosphate and potassium nitrate supported highest activity of Lignin peroxidase whereas best Manganese peroxidase activity was exhibited with ammonium oxalate and laccase with sodium nitrate. The fungus did not express ligninolytic enzymes activity with any organic nitrogen source.

KEYWORDS
Dictyoarthrinium, Chlamydospores, Glucose-peptone Medium, Organic Nitrogen Source

Cite This Paper in IEEE or APA Citation Styles
(a). IEEE Format:
[1] Prasher IB , Chauhan R , "Effect of Carbon and Nitrogen Sources on the Growth, Reproduction and Ligninolytic Enzymes Activity of Dictyoarthrinium Synnematicum Somrith," Advances in Zoology and Botany, Vol. 3, No. 2, pp. 24 - 30, 2015. DOI: 10.13189/azb.2015.030203.

(b). APA Format:
Prasher IB , Chauhan R (2015). Effect of Carbon and Nitrogen Sources on the Growth, Reproduction and Ligninolytic Enzymes Activity of Dictyoarthrinium Synnematicum Somrith. Advances in Zoology and Botany, 3(2), 24 - 30. DOI: 10.13189/azb.2015.030203.