Advances in Zoology and Botany Vol. 3(1), pp. 1 - 5
DOI: 10.13189/azb.2015.030101
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Molecular Genetic Variations among Some Punjab Sheep Breeds Using RAPD Analysis

Hafiz Muhammad Waheed ¹, Safdar Ali ², Muhammad Sajjad Khan ², Muhammad Saif Ur Rehman ², Ghulam Abbas ^3,*
1 Department of Livestock Production & Management, Pir Mehr Ali Shah Arid Agriculture University Rawalpindi
² Department of Animal Breeding & Genetics, University of Agriculture Faisalabad
³ Department of poultry science, University of Agriculture Faisalabad

ABSTRACT

The present study was carried out on seven sheep breeds (Thalli, Lohi, Salt Range, Kajli, Sipli, Buchi and Hissradale) by Random Amplified Polymorphic DNA (RAPD) analysis using 21 random decamer primers. Blood samples were collected from different numbers of animals per breed, of both sexes. After DNA extraction PCR was carried out by using 25 µL reaction mixture containing 3 mM MgCl₂, 100 µM each of dATP, dCTP, dGTP, dTTP, 0.2 µM primer, 15 ng of genomic DNA, and 5 unit of Taq polymerase, through programmable thermal cycler (Crea Con, TCY, USA).Out of 21 decamer primers 16 primers yielded easily scorable bright DNA bands while other five yielded smeared and nonspecific fragments. The genetic similarities of seven sheep breeds were high, ranging from 74.42% to 94.29 %. Thalli and Latti had greatest similarity (94.29%). The lowest genetic similarity was between Lohi and Hissardale (74.42%). The unweighted pair group method of arithmetic means (UPGMA) dendrogram obtained from the cluster analysis revealed two clusters. The study confirms that the molecular genetic techniques such as RAPD-PCR can economically and efficiently be used to establish genetic distances and similarities among and within breeds as well as to find out breed specific genetic markers. The seven sheep breeds in the study could be identified by using one breed specific RAPD marker or a combination of two or more markers. Primer GLB-08 can identify Lohi breed and primer GLA-14 can identify Hissardale. Primer GLA-19 can identify both Kajli and Sipli breeds simultaneously.

KEYWORDS
Sheep, Punjab, Thalli, Lohi, Kajli, RAPD-PCR

Cite This Paper in IEEE or APA Citation Styles
(a). IEEE Format:
[1] Hafiz Muhammad Waheed , Safdar Ali , Muhammad Sajjad Khan , Muhammad Saif Ur Rehman , Ghulam Abbas , "Molecular Genetic Variations among Some Punjab Sheep Breeds Using RAPD Analysis," Advances in Zoology and Botany, Vol. 3, No. 1, pp. 1 - 5, 2015. DOI: 10.13189/azb.2015.030101.

(b). APA Format:
Hafiz Muhammad Waheed , Safdar Ali , Muhammad Sajjad Khan , Muhammad Saif Ur Rehman , Ghulam Abbas (2015). Molecular Genetic Variations among Some Punjab Sheep Breeds Using RAPD Analysis. Advances in Zoology and Botany, 3(1), 1 - 5. DOI: 10.13189/azb.2015.030101.