Universal Journal of Agricultural Research Vol. 2(7), pp. 250 - 252
DOI: 10.13189/ujar.2014.020704
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Effect of Nickel on Male Reproduction


Lukac N. 1,*, Massanyi P. 1, Krockova J. 1, Toman R. 1, Danko J. 2, Stawarz R. 3, Formicki G. 3
1 Department of Animal Physiology, Slovak University of Agriculture in Nitra, Slovakia
2 Institute of Anatomy, University of Veterinary Medicine and Pharmacy in Kosice, Slovakia
3 Institute of Biology, Pedagogical University of Krakow, Poland

ABSTRACT

In this study distribution of nickel as a risk factor of environment in testis and its effects on the testicular structure in experimental animals and effect on spermatozoa was analyzed. In this study the effect of Ni on the testicular structure after an experimental intraperitoneal (i.p.) administration, concentration of nickel in semen of different animal species and the effect of in vitro spermatozoa incubation with nickel on the spermatozoa motility and membrane changes is reported. Our findings clearly suggest a negative effect of nickel on the structure as well as on the function of seminiferous epithelium. In experimental groups with nickel a significant (p<0.001) decrease of germinal epithelium in comparison with control group was observed. The analysis of nickel showed that the concentration of this element in stallion semen was 0.20 mg/kg, in bull 0.12 mg/kg, ram 0.31 mg/kg, boar 0.06 mg/kg and in fox semen 0.36 mg/kg. Concentrations from 125 μM Ni/ml in various time periods of culture stimulate spermatozoa motility after 30 minutes (p<0.001), but later inhibit spermatozoa motility. After a culture of spermatozoa with addition of 125 µM Ni/ml and 240 minutes a typical Annexin–V fluorescence reaction was detected. Fluorescence was detected in mitochondrial segment of bovine spermatozoa. In spermatozoa exposed to higher nickel concentration the Annexin–V positive reaction was detected also on the spermatozoa head membrane. Nickel in very low concentrations (7.8 μM Ni/ml) stimulates the spermatozoa motility but in higher concentrations (>250 μM Ni/ml) cause decrease of spermatozoa motility in vitro.

KEYWORDS
Nickel, Spermatozoa, Testis, Semen

Cite This Paper in IEEE or APA Citation Styles
(a). IEEE Format:
[1] Lukac N. , Massanyi P. , Krockova J. , Toman R. , Danko J. , Stawarz R. , Formicki G. , "Effect of Nickel on Male Reproduction," Universal Journal of Agricultural Research, Vol. 2, No. 7, pp. 250 - 252, 2014. DOI: 10.13189/ujar.2014.020704.

(b). APA Format:
Lukac N. , Massanyi P. , Krockova J. , Toman R. , Danko J. , Stawarz R. , Formicki G. (2014). Effect of Nickel on Male Reproduction. Universal Journal of Agricultural Research, 2(7), 250 - 252. DOI: 10.13189/ujar.2014.020704.