Advances in Pharmacology and Pharmacy Vol. 14(2), pp. 248 - 255
DOI: 10.13189/app.2026.140211
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Olea europaea Leaves Extract: A Guard against Doxorubicin-Induced Cardiotoxicity

Talha Bin Fayyaz ¹, Noor-ul-Ain ², Uzair Nisar ¹, Shakeel Ijaz ^3,*, Raja Anees Taj ³, Aousaf Ahmad ⁴, Muhammad Adeel Arshad ⁵, Sidra Huma ^6
1 Department of Pharmacology, Faculty of Pharmacy, Ziauddin University, Pakistan
² Department of Pharmacology, Institute of Pharmaceutical Sciences, Jinnah Sindh Medical University, Karachi, Pakistan
³ Department of Pharmacy, The University of Lahore, Sargodha Campus, Pakistan
⁴ Quaid-e-Azam College of Pharmacy, Pakistan
⁵ Department of Pharmaceutical Chemistry, Faculty of Pharmaceutical and Allied Health Science, Lahore College for Women University, Pakistan
⁶ Bahawalpur College of Pharmacy, Bahawalpur Medical and Dental College, Pakistan

ABSTRACT

Background: Doxorubicin (DX) is a very potent chemotherapeutic agent, widely used against a variety of malignancies. However, it is associated with a number of organ toxicities i.e. cardiotoxicity, which tend to limit its use in cancer treatment. Recently, plant secondary metabolites have investigated for their chemo-preventive, anticancer as well as cardio-protective potentials. Aim: The current study was designed for the investigation of in-vivo cardio-protective potential of Olea europaea (OE) leaves extract against DX-induced cardiotoxicity in animal models. Methods: Eighteen male Wistar rats in three groups were employed in the study; the control group received only saline, DX group received DX (cumulative dose of 15 mg/kg for one week), while the DX+OE group received DX (15 mg/kg for one week) and OE leaves extract (200mg/kg for six weeks). The assessment methods involved estimation of serum biomarkers for cardiac toxicity including Troponin-I, CPK, CKMB, LDH, TC, TL, TGS, HDL, LDL, and transaminases (AST and ALT) and histopathological examination. Results: Results showed an ample amount of phenolic and flavonoid content in OE extract with promising antioxidant potential. The levels of Troponin-I, CPK, CKMB, LDH, TC, TL, TGS, LDL, and transaminases were significantly reduced in DX-OE pretreated group. This significant reduction in DX-induced increased levels of serum cardiac biomarkers prevented devastating effects on cardiac muscles which was also manifested by histopathological investigations. Conclusion: Conclusively, OE extract remarkably produced a characteristic protective effect on DX-induced cardiotoxicity by suppressing oxidative stress and apoptosis. OE extract can be a potential candidate for combination with DX to mitigate its cardiotoxicity in its long-term clinical use.

KEYWORDS
Cardiotoxicity, Doxorubicin, Olea europaea, Oxidative Stress, Serum Biomarkers

Cite This Paper in IEEE or APA Citation Styles
(a). IEEE Format:
[1] Talha Bin Fayyaz , Noor-ul-Ain , Uzair Nisar , Shakeel Ijaz , Raja Anees Taj , Aousaf Ahmad , Muhammad Adeel Arshad , Sidra Huma , "Olea europaea Leaves Extract: A Guard against Doxorubicin-Induced Cardiotoxicity," Advances in Pharmacology and Pharmacy, Vol. 14, No. 2, pp. 248 - 255, 2026. DOI: 10.13189/app.2026.140211.

(b). APA Format:
Talha Bin Fayyaz , Noor-ul-Ain , Uzair Nisar , Shakeel Ijaz , Raja Anees Taj , Aousaf Ahmad , Muhammad Adeel Arshad , Sidra Huma (2026). Olea europaea Leaves Extract: A Guard against Doxorubicin-Induced Cardiotoxicity. Advances in Pharmacology and Pharmacy, 14(2), 248 - 255. DOI: 10.13189/app.2026.140211.