Advances in Pharmacology and Pharmacy Vol. 14(2), pp. 163 - 174
DOI: 10.13189/app.2026.140204
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Evaluation of Anxiolytic and Antidepressant Activities of the Flavonoid Rich Extract of Leaves of Cassia obtusifolia Linn on Mice

Shailesh M. Kewatkar ^1,*, Ashish D. Tale ², Abhijit S. Khot ², Dipak V. Bhusari ³, Vaishnavi P. Patil ², Satish P. Shelke ⁴, Shirish P. Jain ², Rohit P. Salkute ^5
1 Department of Pharmacognosy, Rajarshi Shahu College of Pharmacy, Amravati University, India
² Department of Pharmacology, Rajarshi Shahu College of Pharmacy, Amravati University, India
³ Department of Pharmaceutics, Rajarshi Shahu College of Pharmacy, Amravati University, India
⁴ Department of Pharmaceutical Quality Assurance, Rajarshi Shahu College of Pharmacy, Amravati University, India
⁵ Department of Allied Sciences, Rajarshi Shahu College of Pharmacy, Amravati University, India

ABSTRACT

Background: The purpose of this study is to assess how Cassia obtusifolia Linn affects the anxiety and depressive-like behaviors, on the other hand, behavioural parameters were assessed without inducing stress. In this study, we use proven models such as brain oxidative indicators, EPM, LDB, anoxic tolerance test for anxiolytic effects and FST & TST for antidepressant effects, antioxidants, and histological observation in conjunction with animal models to evaluate the efficacy of test drugs, i.e., in treating anxiety and depression generated by exposure to stress in mice. Results: The test -1 group received a low dose of COE 100 mg/kg, while the test -2 group received an oral median dose of COE 200 mg/kg, and the test- 3 group received an oral higher dose of COE 300 mg/kg. When COE was administered orally, there was a noticeable and dose-dependent increase in both the amount of time spent in the light chamber and the number of entries. Furthermore, COE exhibits a dose-dependent action that significantly increases the time spent in open arm and no. of entries in open arm. However COE exhibits a dose-dependent effect that considerably increases the latency to convulsion. However COE shortens the immobility duration in FST & TST respectively. However, test drugs show a decrease in LPO levels at lower doses. COE, on the other hand, was discovered to boost the repair of GSH at median dose, and CAT levels at higher doses. Conclusion: Based on the behavioral study, oxidative parameters, and histological observation data, COE significantly improves anxiety and depression-like behavior in experimental mice.

KEYWORDS
Anxiety, Depression, Cassia obtusifolia, Diazepam, EPM, LDB, TST, FST

Cite This Paper in IEEE or APA Citation Styles
(a). IEEE Format:
[1] Shailesh M. Kewatkar , Ashish D. Tale , Abhijit S. Khot , Dipak V. Bhusari , Vaishnavi P. Patil , Satish P. Shelke , Shirish P. Jain , Rohit P. Salkute , "Evaluation of Anxiolytic and Antidepressant Activities of the Flavonoid Rich Extract of Leaves of Cassia obtusifolia Linn on Mice," Advances in Pharmacology and Pharmacy, Vol. 14, No. 2, pp. 163 - 174, 2026. DOI: 10.13189/app.2026.140204.

(b). APA Format:
Shailesh M. Kewatkar , Ashish D. Tale , Abhijit S. Khot , Dipak V. Bhusari , Vaishnavi P. Patil , Satish P. Shelke , Shirish P. Jain , Rohit P. Salkute (2026). Evaluation of Anxiolytic and Antidepressant Activities of the Flavonoid Rich Extract of Leaves of Cassia obtusifolia Linn on Mice. Advances in Pharmacology and Pharmacy, 14(2), 163 - 174. DOI: 10.13189/app.2026.140204.