Environment and Ecology Research Vol. 12(2), pp. 163 - 171
DOI: 10.13189/eer.2024.120207
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Preliminary Screening, Isolation, and Identification of Methylene Blue Dye Degrading Microorganisms from Textile Effluent Contaminated Site

Sasireka Rajendran ^1,*, Gangadharan Muthukumaragurupandian ¹, Kannan Ramesh ¹, Vinoth Rathinam ^2
1 Department of Biotechnology, Mepco Schlenk Engineering College, Sivakasi, Tamilnadu, India
² Department of Electronics and Communication Engineering, P.S.R. Engineering College, Sivakasi, Tamilnadu, India

ABSTRACT

The majority of substances preferred in the food, plastics, printing ink, pharmaceutical, textile, and paper industries are dyes; they impart color to a substrate through a mechanism that can be temporary or permanent. Due to ineffective disposal methods, dyeing operations produce 2,00,000 tons of dye effluents annually, particularly if we concentrate on the textile sector. Hazardous chemicals and other heavy metals found in dyes released as effluent are toxic to people, plants, and other living things. Artificial azo dyes are utilized in many sectors, including the cosmetics, textile, and pharmaceutical sectors. Since these dyes are harmful to humans, getting rid of them is essential. Using microbiological-based dye degradation can be a more efficient and promising approach than using conventional techniques. To degrade the azo dye methylene blue, the current study intends to screen and isolate microbial strains from soil contaminated by textile effluent. More identification and characterization of the isolated microorganisms were done. Based on the phylogenetic analysis, it was observed that strains isolated from contaminated sites were identified as Serratia marcescens. In addition, the isolated strains dye decolorization efficiency was examined. Based on the results it was clear that the strain exhibited a decolorization efficiency of 95 % upon 5 hours of incubation time under dynamic conditions, and elicited better degradation at pH 7 and a temperature of 50 ℃ and a dye concentration of 100 ppm.

KEYWORDS
Textile Effluent, Synthetic Dye, Microbial Degradation, Phylogenetic Analysis, Methylene Blue

Cite This Paper in IEEE or APA Citation Styles
(a). IEEE Format:
[1] Sasireka Rajendran , Gangadharan Muthukumaragurupandian , Kannan Ramesh , Vinoth Rathinam , "Preliminary Screening, Isolation, and Identification of Methylene Blue Dye Degrading Microorganisms from Textile Effluent Contaminated Site," Environment and Ecology Research, Vol. 12, No. 2, pp. 163 - 171, 2024. DOI: 10.13189/eer.2024.120207.

(b). APA Format:
Sasireka Rajendran , Gangadharan Muthukumaragurupandian , Kannan Ramesh , Vinoth Rathinam (2024). Preliminary Screening, Isolation, and Identification of Methylene Blue Dye Degrading Microorganisms from Textile Effluent Contaminated Site. Environment and Ecology Research, 12(2), 163 - 171. DOI: 10.13189/eer.2024.120207.