Advances in Zoology and Botany Vol. 12(3), pp. 151 - 159
DOI: 10.13189/azb.2024.120301
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Exposure to 17α-Ethinylestradiol Alters Brain Histology and Behavioural Response in Fish, Channa punctatus (Bloch. 1793)

Zainab Khatoon ¹, Nooreen Fatima ¹, Sunil P. Trivedi ², Vivek Kumar ^1,*
1 Department of Zoology, Isabella Thoburn College, India
² Department of Zoology, University of Lucknow, India

ABSTRACT

One of the key ingredients in oral contraceptives and the most extensively researched endocrine disruptor globally is 17α-ethynylestradiol (EE2), a synthetic estrogen. The primary way it gets into the environment is via wastewater discharges. In this work, Channa punctatus, a freshwater vertebrate model fish, was used to examine the potential effects of ecologically relevant concentrations of EE2 on behaviour and brain histology. Fish that had been laboratory acclimated and were in good condition were split into three groups and given medium treatment exposure to one treatment at each concentration (5, 10, and 20 ng/L of EE2) for 28 days along with the controls in order to study the potential biological pathways. When the concentration of EE2 was raised after 28 days of exposure, there was an increase in structural damage to the brain. The degradation of brain cells with cytoplasmic vacuolization, alterations in the quantity of grey and white matter, and neuronal necrosis were also noted. The Group 4 (20 ng/L) group showed the worst damage to the brain tissues of both males and females. Male brains have the highest levels of necrosis (22.23±0.54) and vacuolization (16.37±0.44), while female brains exhibit lower levels of necrosis (13.73±0.24) and vacuolization (6.3±0.24), respectively. When exposed to 17α-ethinylestradiol, the test fish displayed various unusual behaviours, including restlessness, asymmetrical swimming movements, loss of balance, motionlessness, and sluggish movement. When exposed to 17α-ethynylestradiol, Channa punctatus internal brain structure had severe histopathological abnormalities, and its behaviour responded differently from that of control fish, demonstrating the severity of synthetic hormones.

KEYWORDS
Channa punctatus, Brain Histology, 17α-Ethinylestradiol, Behaviour

Cite This Paper in IEEE or APA Citation Styles
(a). IEEE Format:
[1] Zainab Khatoon , Nooreen Fatima , Sunil P. Trivedi , Vivek Kumar , "Exposure to 17α-Ethinylestradiol Alters Brain Histology and Behavioural Response in Fish, Channa punctatus (Bloch. 1793)," Advances in Zoology and Botany, Vol. 12, No. 3, pp. 151 - 159, 2024. DOI: 10.13189/azb.2024.120301.

(b). APA Format:
Zainab Khatoon , Nooreen Fatima , Sunil P. Trivedi , Vivek Kumar (2024). Exposure to 17α-Ethinylestradiol Alters Brain Histology and Behavioural Response in Fish, Channa punctatus (Bloch. 1793). Advances in Zoology and Botany, 12(3), 151 - 159. DOI: 10.13189/azb.2024.120301.