Advances in Pharmacology and Pharmacy Vol. 12(2), pp. 104 - 115
DOI: 10.13189/app.2024.120203
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Hypo-testicular Efficacy Assessment of H₂O-methanol (60:40) Extract of Tinospora cordifolia (Willd.) Stem in Albino Rat: A Dose-dependent Study

Puja Das ¹, Dipanwita Mitra ², Dibya Pal ², Debidas Ghosh ^2,*
1 Centre for Life Sciences, Vidyasagar University, India
² Molecular Medicine, Nutrigenomics and Public Health Research Laboratory, Department of Bio-Medical Laboratory Science and Management, Vidyasagar University, India

ABSTRACT

Background: Tinospora cordifolia (Willd.) Miers. traditionally familiar as giloy or gulancha has been used as a traditional folk medicine in Ayurveda from ancient times due to its antifertility effect. However, the mechanisms behind the hypo-testicular action of this herbal plant remain unclear. In this context, this investigation has been performed to unfold the probable mechanism of action by an effective dose of the H₂O-methanol (60:40) extract (HME) of T. cordifolia stem. Objective: To determine the effective dose of T. cordifolia's HME for hypo-testicular efficacy testing in male albino rats. Materials and methods: Doses of T. cordifolia's HME at 5 (low), 10 (moderate), and 20 (high) mg/100 g of body weight (BW)/day for 28 days were given to the experimental rats in three separate dose-treated groups by oral gavage feeding. Rats in the vehicle-treated control group (VTCG) were fed only 0.5 ml sterile water/100 g of BW/day. Spermiological profile, seminal vesicular fructose level, androgenic markers, oxidative stress parameters, and testicular histological studies were analyzed. Results: Different doses of HME-treated groups (HMETGs) delineated a downward deviation significantly (p<0.05) in reproductive organo-somatic indices, spermiological profile, serum testosterone level, androgenic key enzyme’s activities, seminal vesicular fructose level, antioxidant enzyme activity, the diameter of the seminiferous tubules (STD), mitotic and meiotic generations of spermatogenic cell counts at VII^th stage of the spermatokinetics in respect to VTCG. Testicular thiobarbituric acid reactive substances (TBARS) and cholesterol levels were elevated significantly (p<0.05) in the HMETGs in contrast to VTCG. After comparing the HMETGs to VTCG, the changes were noted in toxicity markers at no significant level (p>0.05). Conclusion: The observation enlightens the HME of T. cordifolia stem at the dose of 10 mg (moderate) which executes maximum hypo-testicular activities for male contraception development as well as it also highlighting the therapeutic window of HME in this concern.

KEYWORDS
Tinospora cordifolia, Hypo-Testicular Activity, Spermiological Profile, Androgenic Key Enzymes, Oxidative Stress

Cite This Paper in IEEE or APA Citation Styles
(a). IEEE Format:
[1] Puja Das , Dipanwita Mitra , Dibya Pal , Debidas Ghosh , "Hypo-testicular Efficacy Assessment of H₂O-methanol (60:40) Extract of Tinospora cordifolia (Willd.) Stem in Albino Rat: A Dose-dependent Study," Advances in Pharmacology and Pharmacy, Vol. 12, No. 2, pp. 104 - 115, 2024. DOI: 10.13189/app.2024.120203.

(b). APA Format:
Puja Das , Dipanwita Mitra , Dibya Pal , Debidas Ghosh (2024). Hypo-testicular Efficacy Assessment of H₂O-methanol (60:40) Extract of Tinospora cordifolia (Willd.) Stem in Albino Rat: A Dose-dependent Study. Advances in Pharmacology and Pharmacy, 12(2), 104 - 115. DOI: 10.13189/app.2024.120203.