Advances in Zoology and Botany Vol. 12(1), pp. 13 - 25
DOI: 10.13189/azb.2024.120102
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Determination of Activities of Eucalyptus Leaves Oil and Multiple Extracts on Growth Inhibition of Gram Negative (P. Aeruginosa, E. Coli, and K. Klebsiella Pneumonia), Gram Positive (B. Subtilis & S. Aureus), and Fungi (A. Parasiticus & M. Ramamnianus) Mi


Girish Kumar Vyas 1,*, Hariom Sharma 1, Bhupendra Vyas 2
1 School of Pharmaceutical Studies, Faculty of Health Sciences, Dr. K. N. Modi University, India
2 Department of Pharmacognosy, B. N. Institute of Pharmaceutical Sciences, Bhupal Nobles' University Udaipur, India

ABSTRACT

Background of the research: Eucalyptus is a genus and a member of Myrtaceae family. More than 700 various species are available for this genus. In ancient time, it was widely used for treatment of diseases caused by microbes and bacteria. As it has a good ability to suppress and kill microorganisms, it is widely used in formulation of cosmetics. It was proved by many researchers that its oil contains 1,8-cineole which is used in pulmonary infection treatments. Eucalyptus also showed activities such as antioxidant, antiseptic, anti-inflammatory, herbicidal, insecticidal, anthelmintics. Purpose: The main purpose of this study is to confirm the antimicrobial activity of various concentrations of eucalyptus oil and its extracts in different solvent systems according to the polarity of solvents. As activity against of microbes depends on the presence of antioxidants and less studies were present on antimicrobial effects for selected microbes. Methodologies: Eucalyptus leaves were collected, dried under shades for 48 hours then eucalyptus oil was extracted with Clevenger apparatus by fixing with condenser. The process of extraction was completed with soxhlation process with multiple solvents according to polarity. Then phytochemical evaluation is done for confirmation of antioxidants. Free Radical Scavenging Activity DPPH Test, ABTS Radical-Scavenging Assay, Tannin, Flavonoids, and total Anthocyanin content were determined for good activity. Antimicrobial activity was determined by Disc-Diffusion Assay after calculating the MIC with agar dilution method. Results: The results of using the extract on microbes were found as expected. Eucalyptus oil and extract both were found enriched with antimicrobial properties. These showed good effects against positive, negative bacteria as well as for Fungi. As high antioxidant activity was detected in ethanol extract. Antimicrobial activity results showed that K. pneumoniae seems to be the most sensitive bacteria among both grams (positive and negative bacteria's). The MIC in gram-positive bacteria for S. aureus found 2.85 mg/ml, best in gram negative is K. pneumoniae that is 2.05 mg/ml and for Fungi best MIC is 3.10 mg/ml. Conclusion: These research findings lead us to conclude that eucalyptus essential oil and various extracts, mainly the ethanolic extract could be considered as a potential alternative for synthetic bactericides. It can be used in the pharmaceutical industry for the prevention of pathogenesis caused by microorganisms and free radicals.

KEYWORDS
Antioxidants, DPPH Test, ABTS Test, Phytochemicals, Bacteria, MIC

Cite This Paper in IEEE or APA Citation Styles
(a). IEEE Format:
[1] Girish Kumar Vyas , Hariom Sharma , Bhupendra Vyas , "Determination of Activities of Eucalyptus Leaves Oil and Multiple Extracts on Growth Inhibition of Gram Negative (P. Aeruginosa, E. Coli, and K. Klebsiella Pneumonia), Gram Positive (B. Subtilis & S. Aureus), and Fungi (A. Parasiticus & M. Ramamnianus) Mi," Advances in Zoology and Botany, Vol. 12, No. 1, pp. 13 - 25, 2024. DOI: 10.13189/azb.2024.120102.

(b). APA Format:
Girish Kumar Vyas , Hariom Sharma , Bhupendra Vyas (2024). Determination of Activities of Eucalyptus Leaves Oil and Multiple Extracts on Growth Inhibition of Gram Negative (P. Aeruginosa, E. Coli, and K. Klebsiella Pneumonia), Gram Positive (B. Subtilis & S. Aureus), and Fungi (A. Parasiticus & M. Ramamnianus) Mi. Advances in Zoology and Botany, 12(1), 13 - 25. DOI: 10.13189/azb.2024.120102.