Universal Journal of Agricultural Research Vol. 11(6), pp. 948 - 954
DOI: 10.13189/ujar.2023.110603
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Karamunting (Rhodomyrtus tomentosa) Leaf Extract Inhibits the Growth of Vibrio sp. Isolated from Pacific White-leg Shrimp (Litopenaeus vannamei)

Ni Putu Sinta Puspa Dewi ¹, Retno Kawuri ², Made Pharmawati ³, Pande Gde Sasmita Julyantoro ^4,*
1 Master Programme of Biology, Faculty of Mathematics and Natural Science, Udayana University, Indonesia
² Lab of Microbiology, Biology Study Programme, Faculty of Mathematics and Natural Science, Udayana University, Indonesia
³ Lab of Genetics, Biology Study Programme, Faculty of Mathematics and Natural Science, Udayana University, Indonesia
⁴ Faculty of Marine Science and Fisheries, Udayana University, Indonesia

ABSTRACT

The Pacific White Leg Shrimp (Litopenaeus vannamei) is an introduced shrimp species in Indonesia that faces some cultivation obstacles, such as infection of vibriosis disease caused by Vibrio spp. This study aimed to isolate Vibrio spp. from L. vannamei shrimp, then test the capability of Karamunting (Rhodomyrtus tomentosa) leaf extract for inhibiting the Vibrio isolate by minimum inhibitory concentration (MIC) assays and challenge test toward L. vannamei shrimp. Vibrio identification was carried out by sequencing using the 16S rDNA gene, and MIC tests were performed using ethanol extract of Karamunting leaves through the diffusion well method. The extract was also applied to the shrimp feed for in vivo experiment. The molecular identification showed that the Vibrio isolate has a similarity to Vibrio azureus B12-3, and in vitro experiment resulted in the MIC value of the extract against V. azureus was 3%. Finally, the addition of 100 ppm Karamunting extract to the shrimp feed increases the shrimp survival up to 80% and is significantly different (p<0,05) compared to the control when challenged with V. azureus. This study indicated that the Karamunting (R. tomentosa) has the potency to be a promising natural product to protect L. vannamei shrimp from Vibrio infection.

KEYWORDS
Extract, Karamunting, Shrimp, Vibrio

Cite This Paper in IEEE or APA Citation Styles
(a). IEEE Format:
[1] Ni Putu Sinta Puspa Dewi , Retno Kawuri , Made Pharmawati , Pande Gde Sasmita Julyantoro , "Karamunting (Rhodomyrtus tomentosa) Leaf Extract Inhibits the Growth of Vibrio sp. Isolated from Pacific White-leg Shrimp (Litopenaeus vannamei)," Universal Journal of Agricultural Research, Vol. 11, No. 6, pp. 948 - 954, 2023. DOI: 10.13189/ujar.2023.110603.

(b). APA Format:
Ni Putu Sinta Puspa Dewi , Retno Kawuri , Made Pharmawati , Pande Gde Sasmita Julyantoro (2023). Karamunting (Rhodomyrtus tomentosa) Leaf Extract Inhibits the Growth of Vibrio sp. Isolated from Pacific White-leg Shrimp (Litopenaeus vannamei). Universal Journal of Agricultural Research, 11(6), 948 - 954. DOI: 10.13189/ujar.2023.110603.