Advances in Zoology and Botany Vol. 11(4), pp. 309 - 315
DOI: 10.13189/azb.2023.110408
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Teratogenic Impacts of Tobacco Specific Carcinogen 4-(Methylnitrosamino)-1-(3-Pyridyl)-1-Butanone (NNK) in Swiss Albino Mice Exposed during Gestational Period

Deepshikha Moran ¹, Uma Dutta ^2,*
1 Department of Zoology, Dibru College, India
² Department of Zoology, Cell and Molecular Biology Laboratory, Cotton University, India

ABSTRACT

Marketed tobacco products, both smoking as well as non-smoking, contain pro-carcinogenic component 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK) in high quantities. NNK when ingested, gets activated by action of Cytochrome P450 (CYP450) enzyme present inside living system. In presence of CYP450 enzyme, NNK forms active metabolites NNAL and NNAL-glucs. These metabolites are responsible for adduct formation with DNA and proteins, in turn causing mutation and cancer induction. This study aims at teratogenic effects of tobacco carcinogen NNK on offspring of pregnant Swiss albino mice when exposed to it during gestational period. In this study we have injected a single dose of 100mg/Kg B.wt./ml normal saline of NNK in 6 pregnant dams on 14th day of gestation to find out the embryotoxic and teratogenic effects induced by it. Body weight as well as deformities of the litters produced were recorded. The dams delivered stillbirths with various malformations viz. ascites, ring haemorrhage on tail base, cleft palate, exophthalmia etc. The survived pups developed external tumours on different parts of the body. This indicates that NNK can cross the transplacental barrier between mother and the foetus to induce toxic stress in foetal tissue. It disturbs the smooth progression of the period of organogenesis, as a result initiating various organ deformities. Further extensive studies are required to understand how NNK plays a role in producing teratogenic effects in offspring of mothers exposed to it either active or passive way.

KEYWORDS
NNK, Carcinogen, Teratogenic Impacts, Transplacental Crossing, Gestational Period

Cite This Paper in IEEE or APA Citation Styles
(a). IEEE Format:
[1] Deepshikha Moran , Uma Dutta , "Teratogenic Impacts of Tobacco Specific Carcinogen 4-(Methylnitrosamino)-1-(3-Pyridyl)-1-Butanone (NNK) in Swiss Albino Mice Exposed during Gestational Period," Advances in Zoology and Botany, Vol. 11, No. 4, pp. 309 - 315, 2023. DOI: 10.13189/azb.2023.110408.

(b). APA Format:
Deepshikha Moran , Uma Dutta (2023). Teratogenic Impacts of Tobacco Specific Carcinogen 4-(Methylnitrosamino)-1-(3-Pyridyl)-1-Butanone (NNK) in Swiss Albino Mice Exposed during Gestational Period. Advances in Zoology and Botany, 11(4), 309 - 315. DOI: 10.13189/azb.2023.110408.