Universal Journal of Agricultural Research Vol. 11(4), pp. 673 - 679
DOI: 10.13189/ujar.2023.110401
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Fatty Acid Increment during Senescence of Stored Cabbage: A Metabolomic Approach

Daimon Syukri ^1,*, Kohei Nakano ^2
1 Department of Food and Agricultural Product Technology, Faculty of Agricultural Technology, Andalas University, Indonesia
² The United Graduate School of Agricultural Science, Gifu University, Japan

ABSTRACT

Freshness marker identification is an effort to create a simple system to indicate the freshness level of fresh produces. Freshness is generally related to the degree of senescence that reflects the membrane's lipid turnover, such as phospholipids and fatty acids in the fresh produces. In this study, a targeted analysis of phospholipids and fatty acids in stored cabbage for different duration was conducted by using liquid chromatography-tandem mass spectrometry (LC-MS/MS) for the purpose of identifying the freshness marker metabolites. Cabbage was used due to the specific characteristic as a multy-layer vegetable. Cabbage samples were freshly harvested and immediately stored for 2 and 4 days at 20℃. Lipids for the first layer of cabbage were extracted with methyl tert-butyl ether and subsequently transferred to a LC-MS/MS triple quadrupole linear ion trap system. The multiple reaction monitoring (MRM) program for 240 phospholipids and fatty acids was prepared and utilized for a highly sensitive detection of lipids. Peak area of MRM chromatogram for each detected lipid was calculated statistically analyzed using principal component analysis. The distributions of lipids were separated clearly according to storage duration where phophatidyletanolamines were decreased and accumulation of fatty acid 18:1 was significantly observed during storage. Further study is needed to confirm the accumulation of fatty acid 18:1 could be a potential freshness marker metabolite of stored cabbage through the quantitative determination. Moreover, the selection of another type of fresh produce as the sample is needed to enrich the data for discovery universal freshness marker.

KEYWORDS
Freshness Marker, Lipid Oxidation, Metabolomics, Multi-Layered Vegetable, Phospholipid

Cite This Paper in IEEE or APA Citation Styles
(a). IEEE Format:
[1] Daimon Syukri , Kohei Nakano , "Fatty Acid Increment during Senescence of Stored Cabbage: A Metabolomic Approach," Universal Journal of Agricultural Research, Vol. 11, No. 4, pp. 673 - 679, 2023. DOI: 10.13189/ujar.2023.110401.

(b). APA Format:
Daimon Syukri , Kohei Nakano (2023). Fatty Acid Increment during Senescence of Stored Cabbage: A Metabolomic Approach. Universal Journal of Agricultural Research, 11(4), 673 - 679. DOI: 10.13189/ujar.2023.110401.