Advances in Zoology and Botany Vol. 11(5), pp. 399 - 407
DOI: 10.13189/azb.2023.110508
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The Effect of Pro-oxidant, Anti-oxidant and Anti-Cancerous Drug on Colchicine Induced Polyploidy Cells of Grass Pea Seedling


Aveek Samanta 1, Tilak Raj Maity 2, Shatarupa Dey 2, Suvroma Gupta 2, Siraj Datta 2,3,*
1 Department of Botany, Prabhat Kumar College, India
2 Department of Biotechnology, Haldia Institute of Technology, India
3 Subarnarekha Mahavidyalaya, Gopiballavpur, India

ABSTRACT

In an attempt to correlate polyploidy in grass pea and tumorigenicity, the effects of pro-oxidant, antioxidant and anti-cancerous drugs are evaluated. An increase in H2O2 content and lipid peroxidase (LPx) activity is associated with the stress along with the hike in the antioxidant enzyme levels in colchicine-induced polyploidy. However, ascorbic acid (AA) and methotrexate (MTX) cure oxidative damage owing to their free radical scavenging role and cell division arresting functionality and restoring the cells from polyploidy. This restoration is marked by a fall in the antioxidant enzyme activity namely catalase (CAT), LPx and superoxide dismutase (SOD). Although polyploidy has been controlled, normal growth is compromised as implied the change in radicle lengths of grass peas in various combinations of AA and MTX treatments. Soaking of seed with H2O2 aggravates stress and transforms polyploid cells into necrotic in the higher dose. This preliminary observation seems to be interesting to extrapolate the potentiality of antioxidants as well as anti-cancer drugs for retrieving cells from induced polyploidy.

KEYWORDS
Ascorbic Acid, Catalase, Lathyrus Sativus L., Methotrexate, Mitotic Index, Peroxidase, Polyploid Cell, Superoxide Dismutase

Cite This Paper in IEEE or APA Citation Styles
(a). IEEE Format:
[1] Aveek Samanta , Tilak Raj Maity , Shatarupa Dey , Suvroma Gupta , Siraj Datta , "The Effect of Pro-oxidant, Anti-oxidant and Anti-Cancerous Drug on Colchicine Induced Polyploidy Cells of Grass Pea Seedling," Advances in Zoology and Botany, Vol. 11, No. 5, pp. 399 - 407, 2023. DOI: 10.13189/azb.2023.110508.

(b). APA Format:
Aveek Samanta , Tilak Raj Maity , Shatarupa Dey , Suvroma Gupta , Siraj Datta (2023). The Effect of Pro-oxidant, Anti-oxidant and Anti-Cancerous Drug on Colchicine Induced Polyploidy Cells of Grass Pea Seedling. Advances in Zoology and Botany, 11(5), 399 - 407. DOI: 10.13189/azb.2023.110508.