Advances in Zoology and Botany Vol. 11(3), pp. 203 - 212
DOI: 10.13189/azb.2023.110306
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Studies on Enzyme - Producing Bacteria in the Digestive System of Starfish Collected from Manapad Estuary

T. Arivukkarasi , T. Selva Mohan ^*
Department of Zoology, Rani Anna Govt. College for Women, Manonmaniam Sundaranar University, India

ABSTRACT

Even though studied in various phyla of oceanic invertebrates, the natural diversity of host-associated microbiota has little been studied in Echinodermata members such as starfish, sea urchins, and sea cucumbers. In the present investigation, a considerable amount of the population of amylase, cellulose, lipase, and protease-producing bacteria were detected in the gut of starfish collected from the Manapad estuary. The examination was carried out by standard microbiological methods with proper selective nutrient media. The present study reveals that the amylase-producing population was high (12 x 10⁴ CFU/ml) in February and the cellulase-producing bacterial population was high (7 x 10⁴ CFU/ml) in February and August. Lipase producing bacterial population was high (4.33 x 10⁴ CFU/ml) in September and August (4 x 10⁴ CFU/ml) and the proteolytic bacterial population was found to be the maximum in February (12.66 x 10⁴ CFU/ml). In the present study, two isolates AK3 and AK8 which produces predominant enzymes are screened and on the nucleotide homology and phylogenetic analysis, the enzyme-producing bacterial strain AK3 was recognized and allotted as Enterococcus faecalis strain TKA2 (GenBank Accession Number: MZ823619). The strain AK8 was identified and assigned as Proteus sp. strain TKA1 (GenBank Accession Number: MZ823617). The attained result delivers scope for applications in the food and pharmaceutical industries.

KEYWORDS
Protoreasterlinckii, Manapad, Enzymes, Enterococcus Faecalis, Proteus Sp.

Cite This Paper in IEEE or APA Citation Styles
(a). IEEE Format:
[1] T. Arivukkarasi , T. Selva Mohan , "Studies on Enzyme - Producing Bacteria in the Digestive System of Starfish Collected from Manapad Estuary," Advances in Zoology and Botany, Vol. 11, No. 3, pp. 203 - 212, 2023. DOI: 10.13189/azb.2023.110306.

(b). APA Format:
T. Arivukkarasi , T. Selva Mohan (2023). Studies on Enzyme - Producing Bacteria in the Digestive System of Starfish Collected from Manapad Estuary. Advances in Zoology and Botany, 11(3), 203 - 212. DOI: 10.13189/azb.2023.110306.