Advances in Zoology and Botany Vol. 11(3), pp. 151 - 157
DOI: 10.13189/azb.2023.110301
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GC-MS Analysis and Antibacterial Effect of Methanol Extract of Pterobryopsis Pilifolia (Dixon) Magil

Meenu Mathew , Abraham Mathew ^*
PG and Research Department of Botany, St. Peter's College, India

ABSTRACT

The aim of the study was to serially extract phytochemicals from Pterobryopsis pilifolia and to determine the antibacterial effect of the methanolic extract. Identification of volatile compounds from the methanolic extract was performed to study the possible compounds that offer antibacterial activity. Pterobryopsis pilifolia collected from Idukki district, Kerala, India was used in the present study. Serial extraction of phytochemicals from the bryophyte was done using Soxhlet apparatus with petroleum ether, followed by ethyl acetate and then methanol as solvent. The methanolic extract was used in the present study. The extract was filtered and concentrated using rotary evaporator. Antibacterial activity was tested against ten standard strains of bacteria. Methanolic extract inhibited all the ten tested strains that include both Gram positive and Gram negative bacteria. The highest zone of inhibition noted was against Xanthomonas campestris with an inhibition zone of 27 mm ± 1 mm. GC-MS analysis of the extract revealed the presence of several volatile compounds including fatty acids and other bioactive compounds. Pterobryopsis pilifolia is usually seen intact without any signs of disease, insects or animals feeding on them. The presence of these compounds might be the reason for the resistance of the taxa against diseases or pests. Studies on these compounds thus can pay the way for elucidating mechanism for pest and disease resistance in plants. The compounds can be used as antifeedant spray that can be applied to crop plants, thereby reducing the effect of harmful chemical pesticides.

KEYWORDS
Bryophyte, Soxhlet Extraction, Pterobryopsis Pilifolia, Methanol, GC-MS

Cite This Paper in IEEE or APA Citation Styles
(a). IEEE Format:
[1] Meenu Mathew , Abraham Mathew , "GC-MS Analysis and Antibacterial Effect of Methanol Extract of Pterobryopsis Pilifolia (Dixon) Magil," Advances in Zoology and Botany, Vol. 11, No. 3, pp. 151 - 157, 2023. DOI: 10.13189/azb.2023.110301.

(b). APA Format:
Meenu Mathew , Abraham Mathew (2023). GC-MS Analysis and Antibacterial Effect of Methanol Extract of Pterobryopsis Pilifolia (Dixon) Magil. Advances in Zoology and Botany, 11(3), 151 - 157. DOI: 10.13189/azb.2023.110301.