Universal Journal of Agricultural Research Vol. 11(2), pp. 425 - 433
DOI: 10.13189/ujar.2023.110219
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Plant-Based Total Protein Determination of Soybeans Using Fluorescent Technique

P. Suwannasopon ^1,2,*, V. Changrue ³, K. Katsri ^4
1 Graduate School, Chiang Mai University, Chiang Mai 50200, Thailand
² Postharvest Technology Research Center, Faculty of Agriculture, Chiang Mai University, Chiang Mai 50100, Thailand
³ Department of Mechanical Engineering, Faculty of Engineering, Chiang Mai University, Chiang Mai 50200, Thailand
⁴ School of Agriculture and Natural Resources, University of Phayao, Phayao 56000, Thailand

ABSTRACT

Due to the time consuming of conventional method in plant-based total protein determination in soybeans, this research aims to find an alternative shorter time technique using fluorescent technique. The first experiment was finding optimum conditions of sodium hydroxide concentration and time on protein extraction in extraction process. In the second experiment, the solution from the best conditions of the first experiment will be added by Coomassie brilliant blue G-250. The complete reaction time will be recorded. In the last step of this study, the fluorescent will be applied to find the responded wavelength of protein-dye-complex. Then, the prediction of overall protein content will be summarized. The results of the studies revealed that optimum of sodium hydroxide concentration was 0.8 % and extraction time was 120 min. In terms of the reaction time of amino with Coomassie brilliant blue G-250 was used completely for 5 min. A modification of the Bradford method revealed a protein-dye-complex at a wavelength of 702.95 nm. The total protein content (y) prediction equation was y = 9.4325x-1.6175, where x value represented the degree of fluorescence intensity. The analysis period of total protein determination using fluorescent technique was 3.5 hours.

KEYWORDS
Fluorescent Technique, Total Protein, Soybean

Cite This Paper in IEEE or APA Citation Styles
(a). IEEE Format:
[1] P. Suwannasopon , V. Changrue , K. Katsri , "Plant-Based Total Protein Determination of Soybeans Using Fluorescent Technique," Universal Journal of Agricultural Research, Vol. 11, No. 2, pp. 425 - 433, 2023. DOI: 10.13189/ujar.2023.110219.

(b). APA Format:
P. Suwannasopon , V. Changrue , K. Katsri (2023). Plant-Based Total Protein Determination of Soybeans Using Fluorescent Technique. Universal Journal of Agricultural Research, 11(2), 425 - 433. DOI: 10.13189/ujar.2023.110219.