Universal Journal of Agricultural Research Vol. 11(2), pp. 266 - 274
DOI: 10.13189/ujar.2023.110205
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The Effects of Metallochlorophyll Formation and Pretreatment on Color, Chlorophyll Content, Total Phenolic Content, and Antioxidant Activity of Sambiloto (Andrographis paniculata) Simplicia Powder

Chatarina Lilis Suryani ^1,*, Ichlasia Ainul Fitri ¹, Elvanita Evlin ¹, Norfakdah ¹, FX. Suwarta ^2
1 Food Technology Study Program, Faculty of Agroindustry, Universitas Mercu Buana Yogyakarta, Yogyakarta, Indonesia
² Animal Husbandry Study Program, Faculty of Agroindustry, Universitas Mercu Buana Yogyakarta, Yogyakarta, Indonesia

ABSTRACT

This research aimed to produce sambiloto simplicia which is rich in chlorophyll and Zn through the process of forming metallochlorophyll by blanching either fresh or dry leaves. Sambiloto simplicia powder is produced through several stages including sorting, boiling in Zn acetate medium (0, 300, 400, 500 ppm), draining, drying, and grinding. The types of materials used are dry and fresh leaves material. The sambiloto simplicia powder was analyzed for chlorophyll content, bound Zn content, total phenolic content (TPC), and total flavonoid content (TFC). Analysis of the antioxidant activity of sambiloto simplicia powder included lipid peroxidation inhibition (LPI) activity and DPPH radical scavenging capacity. The results show that the greater Zn acetate concentration, the green color intensity, chlorophyll content, bound Zn content, and total phenolic and flavonoid content of sambiloto simplicia powder increased, but at concentrations more than 300 ppm in dry leaves and more than 400 ppm in fresh leaves it decreased again. Simplicia powder from dry leaves by blanching at a concentration of 300 ppm and fresh leaves material at a concentration of 400 ppm had the highest contents of chlorophyll, TPC, TFC, and the ability to inhibit lipid peroxidation. At a concentration of Zn acetate of 300 ppm, the simplicia powder from the dry leaves had a higher RSA and LPI than the fresh leaves, but at a concentration of 400 ppm, the LPI from the fresh leaves was higher. The sambiloto simplicia powder could be a natural source of antioxidants as it is high in zinc.

KEYWORDS
Metallochlorophyll, Andrographis paniculata, Phenolics, Flavonoids, Antioxidant Activity

Cite This Paper in IEEE or APA Citation Styles
(a). IEEE Format:
[1] Chatarina Lilis Suryani , Ichlasia Ainul Fitri , Elvanita Evlin , Norfakdah , FX. Suwarta , "The Effects of Metallochlorophyll Formation and Pretreatment on Color, Chlorophyll Content, Total Phenolic Content, and Antioxidant Activity of Sambiloto (Andrographis paniculata) Simplicia Powder," Universal Journal of Agricultural Research, Vol. 11, No. 2, pp. 266 - 274, 2023. DOI: 10.13189/ujar.2023.110205.

(b). APA Format:
Chatarina Lilis Suryani , Ichlasia Ainul Fitri , Elvanita Evlin , Norfakdah , FX. Suwarta (2023). The Effects of Metallochlorophyll Formation and Pretreatment on Color, Chlorophyll Content, Total Phenolic Content, and Antioxidant Activity of Sambiloto (Andrographis paniculata) Simplicia Powder. Universal Journal of Agricultural Research, 11(2), 266 - 274. DOI: 10.13189/ujar.2023.110205.