Advances in Zoology and Botany Vol. 11(2), pp. 121 - 128
DOI: 10.13189/azb.2023.110204
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Evaluation of Acaricidal Activity of Cinnamomum camphora (F. Lauraceae) Essential Oil Nanoemulsion Against Cattle Tick Rhipicephalus microplus

Anuradha Kapoor , Shabad Preet ^*
Department of Zoology, Faculty of Science, Dayalbagh Educational Institute, Agra, 282005 Uttar Pradesh, India

ABSTRACT

The present study aims at preparing nanoemulsions of plant essential oil Cinnamomum camphora as acaricidal agent against cattle tick Rhipicephalus microplus larvae. In the initial step, the phytochemical screening of oil was done through gas chromatography and mass spectrometry depicting major compounds as α-pinene (53.99%) and camphor (20.89%) in C. camphora oil. Different ratios of Cinnamomum camphora oil and surfactant Tween 80 (oil:surfactant- 1:0.25, 1:0.5, 1:1, 1:1.5, 1:2 and 1:2.5) were used for preparing stable nanoemulsion through ultrasonication approach. Thermodynamic stability analysis exhibited that CA3 ratio (1:1) to be the most stable which was further characterized using Dynamic light scattering study through Nano zeta sizer indicating droplet size of 101.60 nm, polydispersity index as 0.50 and zeta potential value measured as -8.59 mV. Spectral analysis through Fourier Transform Infrared spectrometry for functional group interaction and Transmission electron microscopy for morphology of nanoemulsion droplets was also done for CA3 nanoemulsion. Acaricidal activity of stable nanoemulsion was evaluated against R. microplus larvae using larval packet assay and repellency rod test with concentration ranging from 500 ppm to 20000 ppm and 500 ppm to 25000 ppm respectively. Highest concentration (20000 ppm) showed 77% mean mortality with LC₅₀ (3364.46 ppm) after 24 hour. CA3 showed promising repellent activity that ranged from 27% to 87% at various concentrations at different time intervals. These findings proved that Cinnamomum camphor essential oil nanoemulsion could be developed and used against R. microplus larvae as a safer, greener and nanoacaricide product.

KEYWORDS
Cinnamomum camphora Essential Oil, Nanoemulsion, Ultrasonication, Larvae, Rhipicephalus microplus

Cite This Paper in IEEE or APA Citation Styles
(a). IEEE Format:
[1] Anuradha Kapoor , Shabad Preet , "Evaluation of Acaricidal Activity of Cinnamomum camphora (F. Lauraceae) Essential Oil Nanoemulsion Against Cattle Tick Rhipicephalus microplus," Advances in Zoology and Botany, Vol. 11, No. 2, pp. 121 - 128, 2023. DOI: 10.13189/azb.2023.110204.

(b). APA Format:
Anuradha Kapoor , Shabad Preet (2023). Evaluation of Acaricidal Activity of Cinnamomum camphora (F. Lauraceae) Essential Oil Nanoemulsion Against Cattle Tick Rhipicephalus microplus. Advances in Zoology and Botany, 11(2), 121 - 128. DOI: 10.13189/azb.2023.110204.