Bioengineering and Bioscience(CEASE PUBLICATION) Vol. 2(1), pp. 1 - 5
DOI: 10.13189/bb.2014.020101
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Isolation of Cellulose-Degrading Actinomycetes and Evaluation of their Cellulolytic Potential

Yugal Kishore Mohanta ^*
Department of Biotechnology, North Orissa University, Baripada, 757003, India

ABSTRACT

Nine isolates of cellulose-degrading Actinomycetes were isolated from different sediment samples from the Bhitarkanika Mangrove Forest. Cellulase and hydrolytic activity was confirmed with clear zone around the colony on basal salt agar medium using Congo Red. The enzyme assays for two enzymes, filter paper cellulase (FPCase), and carboxymethylcellulase (CMCase), were examined by methods recommended by the International Union of Pure and Applied Chemistry (IUPAC). The extracellular cellulase activities ranged from 0.266±0.001 to 0.734±0.001 IU/mL for FPC and 0.501±0.014 to 1.381±0.024 IU/mL for CMCase or endoglucanase assay.

KEYWORDS
Celluloytic Actinomycetes, FPCase, CMCase, Bhitarkanika Mangrove Forest

Cite This Paper in IEEE or APA Citation Styles
(a). IEEE Format:
[1] Yugal Kishore Mohanta , "Isolation of Cellulose-Degrading Actinomycetes and Evaluation of their Cellulolytic Potential," Bioengineering and Bioscience(CEASE PUBLICATION), Vol. 2, No. 1, pp. 1 - 5, 2014. DOI: 10.13189/bb.2014.020101.

(b). APA Format:
Yugal Kishore Mohanta (2014). Isolation of Cellulose-Degrading Actinomycetes and Evaluation of their Cellulolytic Potential. Bioengineering and Bioscience(CEASE PUBLICATION), 2(1), 1 - 5. DOI: 10.13189/bb.2014.020101.