Journals Information
Universal Journal of Microbiology Research(CEASE PUBLICATION) Vol. 2(1), pp. 15 - 22
DOI: 10.13189/ujmr.2014.020102
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Development of a 16S rRNA PCR-RFLP Assay for Bartonella identification: Applicability in the Identification of Species Involved in Human Infections
Luis J. del Valle 1, Michael L. Jaramillo 2, Miguel Talledo 2, Maria J. Pons 3, Lidia Flores 4, Ruth L. Quispe 4, Pablo Ramírez 4, Ruth García-de-la-Guarda 4, Débora Alvarado 4, Abraham Espinoza-Culupú 4, Juana del Valle 5,6, Martha Vargas 7, Joaquim Ruíz 3,*
1 Centre de Biotecnología Molecular, Departament d’Enginyeria Química, Universitat Politècnica de Catalunya, Barcelona, Spain
2 Laboratorio de Microbiología Molecular y Biotecnología, Facultad de Ciencias Biológicas, Universidad Nacional Mayor de San Marcos, Lima, Perú.
3 Barcelona Centre for International Health Research (CRESIB), Hospital Clinic, Universitat de Barcelona, Spain
4 Laboratorio de Microbiología Molecular y Biotecnología, Facultad de Ciencias Biológicas, Universidad Nacional Mayor de San Marcos, Lima, Perú
5 Universidad Peruana de Ciencias Aplicadas, Lima, Perú
6 Instituto de Investigación Nutricional, Lima, Perú
7 Hospital Clinic de Barcelona, Barcelona, Spain
ABSTRACT
We designed a 16S rRNA gene PCR-RFLP scheme to identify all currently described Bartonella spp. The 16S rRNA genes of all Bartonella spp. were in-silico analyzed in order to design a RFLP technique able to discriminate among different species. The restriction enzymes selected were MaeIII, MseI, Sau96I, BsaAI, DrdI, FokI, BssHII, BstUI, AluI, TspDTI and HphI which, according to a decision-making tree, facilitated the differentiation of all the currently described species of Bartonella.The technique was experimentally tested in different species of Bartonella, including human pathogenic B. bacilliformis and B. henselae with a 100% of concordance with the in-silico predicted patterns.This novel RFLP assay could be used to identify both human and non-human pathogenic Bartonella in diagnostic, phylogenetic and epidemiologic studies.
KEYWORDS
Bartonella, PCR-RFLP, 16s rRNA Gene, Identification
Cite This Paper in IEEE or APA Citation Styles
(a). IEEE Format:
[1] Luis J. del Valle , Michael L. Jaramillo , Miguel Talledo , Maria J. Pons , Lidia Flores , Ruth L. Quispe , Pablo Ramírez , Ruth García-de-la-Guarda , Débora Alvarado , Abraham Espinoza-Culupú , Juana del Valle , Martha Vargas , Joaquim Ruíz , "Development of a 16S rRNA PCR-RFLP Assay for Bartonella identification: Applicability in the Identification of Species Involved in Human Infections," Universal Journal of Microbiology Research(CEASE PUBLICATION), Vol. 2, No. 1, pp. 15 - 22, 2014. DOI: 10.13189/ujmr.2014.020102.
(b). APA Format:
Luis J. del Valle , Michael L. Jaramillo , Miguel Talledo , Maria J. Pons , Lidia Flores , Ruth L. Quispe , Pablo Ramírez , Ruth García-de-la-Guarda , Débora Alvarado , Abraham Espinoza-Culupú , Juana del Valle , Martha Vargas , Joaquim Ruíz (2014). Development of a 16S rRNA PCR-RFLP Assay for Bartonella identification: Applicability in the Identification of Species Involved in Human Infections. Universal Journal of Microbiology Research(CEASE PUBLICATION), 2(1), 15 - 22. DOI: 10.13189/ujmr.2014.020102.