Journals Information
Food Science and Technology Vol. 8(4), pp. 79 - 86
DOI: 10.13189/fst.2020.080402
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Characterization and Properties of Gedi (Abelmoschus Manihot L.) Leaf Extract with Liquid Chromatography Mass Spectrometry Using Quadrupole Time-of-Flight Technology (LCMS-QToF)
Tri Yuni Hendrawati *, Afra Nuraini , Rusnia Junita Hakim , Nurul Hidayati Fithriyah
Department of Chemical Engineering, Faculty of Engineering, Universitas Muhammadiyah Jakarta, Jl. Cempaka Putih Tengah 27, Jakarta, 10510, Indonesia
ABSTRACT
Gedi (Abelmoschus Manihot L.) is a tropical plant in the Malvaceae family, one of the groups of plants in the herbal community. Gedi plants are known to contain flavonoids, hormones, alkaloids, tannins and phenolic compounds. An ultrasonic extraction tool is used in the extraction process of Gedi leaves. The objective of the study is to know the yield and analyze Gedi leaf extract, including proximate analysis and active substance components with LCMS-QTof. The material used is Gedi leaves from Manado. Ultrasonic extraction was carried out with a dry Gedi leaf ratio and Ethanol 1:40 (m/v) with a time of 30 minutes. The concentration of the extract is with a rotary evaporator temperature of 50-60℃. The yield of the extract of Gedi leaf is 9.86%. Study of the proximity of dried Gedi leaf powder revealed 27.135% protein, 41.675% carbohydrate, 329 kcal energy, 11.235% water and 13.83% ash material. Meanwhile for proximity products, the Gedi leaf extract contains 0.635% protein, 1.27% carbohydrate, 9.26% total energy, 7.1% water, and 0.635% ash. The active compounds in the Gedi extract have been analyzed using LCMS. Trigonelline, Gentiatibetine, Harman, Periplocoside C, and Biotin were detected using the positive-ionization mode, whereas Chloramphenicol was detected with negative-ionization mode.
KEYWORDS
Gedi Leaf, Extraction, Ultrasonic, Antioxidant, LCMS-QToF
Cite This Paper in IEEE or APA Citation Styles
(a). IEEE Format:
[1] Tri Yuni Hendrawati , Afra Nuraini , Rusnia Junita Hakim , Nurul Hidayati Fithriyah , "Characterization and Properties of Gedi (Abelmoschus Manihot L.) Leaf Extract with Liquid Chromatography Mass Spectrometry Using Quadrupole Time-of-Flight Technology (LCMS-QToF)," Food Science and Technology, Vol. 8, No. 4, pp. 79 - 86, 2020. DOI: 10.13189/fst.2020.080402.
(b). APA Format:
Tri Yuni Hendrawati , Afra Nuraini , Rusnia Junita Hakim , Nurul Hidayati Fithriyah (2020). Characterization and Properties of Gedi (Abelmoschus Manihot L.) Leaf Extract with Liquid Chromatography Mass Spectrometry Using Quadrupole Time-of-Flight Technology (LCMS-QToF). Food Science and Technology, 8(4), 79 - 86. DOI: 10.13189/fst.2020.080402.