International Journal of Biochemistry and Biophysics Vol. 7(1), pp. 1 - 5
DOI: 10.13189/ijbb.2019.070101
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Evaluation of Antidepressant Activity of Dolichos Biflorus Methanolic Seed Extract in Animal Models of Depression


Mahalaxmi S Petimani , Prabhakar Adake *, Abhilash Aditya
Yenepoya Medical College, India

ABSTRACT

Objective: To evaluate the antidepressant activity of methanolic extract of Dolichos Biflorus seeds in Swiss albino mice. Methodology: A total of 72(n=72) Swiss albino male mice were used in the study. They were divided into 12 groups of six mice in each. First six groups were evaluated by Tail Suspension Test (TST) and remaining by Forced Swim Test (FST). First group of mice (control) received normal saline 10mg/kg, second group (standard) received Imipramine 10mg/kg and third, fourth, fifth and sixth groups (test) respectively received methanolic extract of Dolichos Biflorus seeds (MEDB) 100mg/kg, 200mg/kg, 300mg/kg and 400mg/kg dose orally for 30 days. They were evaluated for antidepressant activity using TST after 60 minutes of drug administration on 30th day. Duration of immobility was noted for six minutes for each mouse in all groups. Similarly, remaining six groups (7th to 12th) received the same drugs and evaluated for antidepressant activity using FST after 60 minutes of drug administration. Results: Results were analyzed by Kruskal-Wallis non-parametric test followed by Dunnet's test for comparison between groups. The immobility periods were expressed in mean±SD. The immobility period in TST were 245±9.7, 80±7.8, 216.4±4.9, 225.3±2.3, 198.4±3.2 and 185.4±2.7 seconds respectively for control, standard and four test groups (100/200/300/400mg/kg). Similarly, immobility periods of 139.33±7.9, 71.66±4.9, 54.7±2.9, 58±5.2, 126.2±7.9 and 113.4±2.8 seconds were noted for FST for remaining six groups of mice. In TST, test drug MEDB in all the doses (50/100/200/300/400mg/day) failed to show statistically significant antidepressant action when compared to control group (p>0.05). Whereas, in FST, MEDB in a dose of 200mg/kg has shown statistically significant (p<0.05) antidepressant action when compared to control. Conclusion: Present study shown methanolic extract of Dolichos Biflorus seeds (MEDB) has significant antidepressant activity at the dose of 200mg/kg in FST model of depression in mice.

KEYWORDS
Methanolic Extract of Dolichos Biflorus Seeds (MEDB), Forced Swim Test, Tail Suspension Test

Cite This Paper in IEEE or APA Citation Styles
(a). IEEE Format:
[1] Mahalaxmi S Petimani , Prabhakar Adake , Abhilash Aditya , "Evaluation of Antidepressant Activity of Dolichos Biflorus Methanolic Seed Extract in Animal Models of Depression," International Journal of Biochemistry and Biophysics, Vol. 7, No. 1, pp. 1 - 5, 2019. DOI: 10.13189/ijbb.2019.070101.

(b). APA Format:
Mahalaxmi S Petimani , Prabhakar Adake , Abhilash Aditya (2019). Evaluation of Antidepressant Activity of Dolichos Biflorus Methanolic Seed Extract in Animal Models of Depression. International Journal of Biochemistry and Biophysics, 7(1), 1 - 5. DOI: 10.13189/ijbb.2019.070101.