International Journal of Neuroscience and Behavioral Science Vol. 5(1), pp. 7 - 18
DOI: 10.13189/ijnbs.2017.050102
Reprint (PDF) (543Kb)


Antidepressant Activity and Mechanism of Aqueous Extract of Vigna Unguiculata ssp. Dekindtiana (L.) Walp Dried Aerial Part in Mice


Lateef Abiola Akinpelu 1, Theophilus Adekunle Adegbuyi 1, Samuel Sunday Agboola 1, Julius Kayode Olaonipekun 1, Idowu Julius Olawuni 2, Adesoji Mutiu Adegoke 3, Idris Ajayi Oyemitan 1,*, Gbola Olayiwola 4
1 Department of Pharmacology, Obafemi Awolowo University, Ile-Ife, Osun State, Nigeria
2 Department of Biochemistry, Obafemi Awolowo University, Ile-Ife, Osun State, Nigeria
3 Central Science Laboratory, Obafemi Awolowo University, Ile-Ife, Osun State, Nigeria
4 Department of Clinical Pharmacy, Faculty of Pharmacy, Obafemi Awolowo University, Ile-Ife, Osun State, Nigeria

ABSTRACT

Objective: Vigna unguiculata ssp. dekindtiana (L.) Walp is used in traditional practice to treat depression-like disorders in some communities of Southwest Nigeria. This study investigated the antidepressant-like effects of the aqueous fraction of the dried aerial parts of V. unguiculata ssp. dekindtiana (AFVU). Methodology: AFVU was evaluated for antidepressant effect on the force-swimming test (FST), tail suspension test (TST) and locomotor activity (LC) in the open field test (OFT) using mice; and its probable neural mechanism(s) investigated using various receptor antagonists. Elemental composition (EC) and phyto-constituents of AFVU were analyzed using standard methods. Results: The AFVU (600 and 800 mg/kg, p.o.) significantly (p<0.05) decreased the immobility time of mice in FST and TST without significant (p<0.05) effect on LC, suggesting that its antidepressant-like effect is specific; anti-immobility effect of AFVU was significantly (p<0.05) blocked by intraperitoneal injection of prazosin (62.5 µg/kg), yohimbine, (1 mg/kg), cyproheptadine (3 mg/kg), sulpiride (50 mg/kg), methylene blue (10 mg/kg) and L-NNA (10 mg/kg) suggesting adrenergic, serotonergic, dopaminergic and nitergic pathways. The EC assured its safety; while phenols and alkaloids were the most abundant phytoconstituents in AFVU. Conclusion: This study concluded that AFVU possessed antidepressant-like effects which may be mediated through multiple receptor pathways.

KEYWORDS
Fabaceae, Atomic Absorption Spectroscopy, Elemental Composition, Phytoconstituents, Forced-swimming Test, Tail Suspension Test, Receptor Antagonists

Cite this paper
Lateef Abiola Akinpelu , Theophilus Adekunle Adegbuyi , Samuel Sunday Agboola , Julius Kayode Olaonipekun , Idowu Julius Olawuni , Adesoji Mutiu Adegoke , Idris Ajayi Oyemitan , Gbola Olayiwola . "Antidepressant Activity and Mechanism of Aqueous Extract of Vigna Unguiculata ssp. Dekindtiana (L.) Walp Dried Aerial Part in Mice." International Journal of Neuroscience and Behavioral Science 5.1 (2017) 7 - 18. doi: 10.13189/ijnbs.2017.050102.