International Journal of Cardiovascular and Cerebrovascular Disease Vol. 5(1), pp. 8 - 14
DOI: 10.13189/ijccd.2017.050102
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Cardio Protective Action of Cilostazol, Milrinone and Their Combination over Isoproterenol Induced Myocardial Infarction in Wistar Rats


Prashant Kumar Dhakad 1,*, Pramod Kumar Sharma 1, Sokindra Kumar 2, Ashish Kr. Sharma 3, Raghav Mishra 1, Aditya Dixit 1
1 Department of Pharmacy, Galgotias University, India
2 R.V. Northland Institute (RVNI), India
3 Department of Pharmacology, NIMS Institute of Pharmacy, NIMS University, India

ABSTRACT

The cardio protective action of cilostazol, milrinone and their combination effect on myocardial infarction induced by isoproterenol subjected to Wistar rats was done by studying various parameters - heart rate, % infarct size change, LDH level, CPKMB level, ECG pattern. In the following research work effect of synthetic drugs cilostazol, milrinone and their combined effect were studied over various biochemical and cardiac parameters. Isoproterenol was used in inducing myocardial infarction to Wistar rats. Isoproterenol (15 mg/100g) administration, causes an increased levels of biochemical markers-lactate dehydrogenase (2546.25±1.38), creatine phosphokinase (1485.5±.80) in serum of rats indicating potential action of isoproterenol on myocardium. Due to necrosis of myocardium a marked increase of these enzymes was seen in blood. A significant decrease in heart rate (262±.96) in isoproterenol treated rats was observed. Infarct size in ISO group rats (41%±.008) increased to certain extent with promiscuous yellow colored non-viable cells visible indicating infarction. Statistical analysis done by one-way ANOVA followed by the Dunnett's multiple comparison test where ISO control group compared with other groups, p value < 0.001 indicating extremely significant results. The present study demonstrated pharmacological benefits of combination dose of cilostazol, milrinone as evidenced by improvement in heart rate, Lactate dehydrogenase level, Creatine phosphokinase MB level, Electrocardiogram pattern, % infarct size.

KEYWORDS
Isoproterenol, Cilostazol, Milrinone, Heart Rate, Infarct Size, Lactate Dehydrogenase, Creatine Phosphokinase

Cite this paper
Prashant Kumar Dhakad , Pramod Kumar Sharma , Sokindra Kumar , Ashish Kr. Sharma , Raghav Mishra , Aditya Dixit . "Cardio Protective Action of Cilostazol, Milrinone and Their Combination over Isoproterenol Induced Myocardial Infarction in Wistar Rats." International Journal of Cardiovascular and Cerebrovascular Disease 5.1 (2017) 8 - 14. doi: 10.13189/ijccd.2017.050102.