International Journal of Biochemistry and Biophysics Vol. 4(4), pp. 37 - 44
DOI: 10.13189/ijbb.2016.040401
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The Role of Microsomal Galactose-tissues Specific Lectins from Prostate in Gland Peroxidation


Nino Kvitsinadze 1, Elene Davitashvili 1,*, Maka Koshoridze 1, Ketevan Chubinidze 1, Nana Koshoridze 1, Mzia Goguadze 2, Omar Tsintsadze 2, Guram Karazanashvili 3, Revaz Solomonia 4
1 Faculty of Exact and Natural Sciences, Department of Biology, I. Javakhishvili Tbilisi State University, Georgia
2 A. Tsulukidze Urology National Center, Pathomorphology Laboratory at the 1st Clinical Hospital, Georgia
3 Clinic of SJC "Modern Medical Technologies", Georgia
4 Institute of Chemical Biology, Ilia State University, Georgia

ABSTRACT

Galactose-specific lectins are proteins with multifunctional properties, modulate cancerogenesis multistep process, and induce the remarkable generation of intracellular hydrogen peroxide. In this data we aim to investigate the effects of galactose-specific lectins isolated from the microsomal fraction of prostate post-operation tissues with different diagnosis in vitro model experiments (bovine liver tissue cells) on some aspects of peroxidation processes as one of the causes of tumor genesis. The beta-galactose-specific lectins were isolated from the microsomal fraction of prostate tissues with different diagnosis: benign prostate hyperplasia with low-grade intraepithelial neoplasia (BPH/LGPIN), high grade intraepithelial neoplasia (HGPIN), atypical adenomatous hyperplasia (AAH) and adenocarcinoma (PC). The molecular weight (60kDa) of lectins found to be similar, but their biological effects are different. The lectin from BPH/LGPIN tissue microsomes did not change the amount of malondyaldehyde (MDA), while the lectin from HGPIN, AAH and PC tissue microsomes induced increasing production of MDA. The lectins from all sources increase the amount of H2O2 in time-dependent manner. These effects are mediated by galactose binding domain of the lectins as are completely abolished by the inclusion of galactose. We proposed that one of the reasons of the revealed changes in the biological properties of lectins induced by alteration in the lectin's carbohydrate moiety structure. There is evidence that the changes of biological properties of prostate galactose-specific lectins are functionally related to development of the metabolic changes during prostate pathogenesis. In view of endoplasmic reticulum in many ways involved in carcinogenesis, we proposed the possible way, the released galactose-specific lectins from damaged microsomes enhances further transformation of prostate tissue by induction of MDA production and generation of free H2O2.

KEYWORDS
Microsomal Lectins, Catalase, Lipid Peroxidation, Malondialdehyde, Prostate Gland Diseases

Cite This Paper in IEEE or APA Citation Styles
(a). IEEE Format:
[1] Nino Kvitsinadze , Elene Davitashvili , Maka Koshoridze , Ketevan Chubinidze , Nana Koshoridze , Mzia Goguadze , Omar Tsintsadze , Guram Karazanashvili , Revaz Solomonia , "The Role of Microsomal Galactose-tissues Specific Lectins from Prostate in Gland Peroxidation," International Journal of Biochemistry and Biophysics, Vol. 4, No. 4, pp. 37 - 44, 2016. DOI: 10.13189/ijbb.2016.040401.

(b). APA Format:
Nino Kvitsinadze , Elene Davitashvili , Maka Koshoridze , Ketevan Chubinidze , Nana Koshoridze , Mzia Goguadze , Omar Tsintsadze , Guram Karazanashvili , Revaz Solomonia (2016). The Role of Microsomal Galactose-tissues Specific Lectins from Prostate in Gland Peroxidation. International Journal of Biochemistry and Biophysics, 4(4), 37 - 44. DOI: 10.13189/ijbb.2016.040401.