International Journal of Biochemistry and Biophysics Vol. 4(2), pp. 16 - 23
DOI: 10.13189/ijbb.2016.040202
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Media Optimization for Exopolysaccharide Producing Klebsiella pneumoniae KU215681 under Varying Cultural Conditions


T. Sivakumar *, T. Shankar , V. Thangapandian , S. Mahendran
Department of Microbiology, Ayya Nadar Janaki Ammal College (Autonomous), India

ABSTRACT

The soil samples were collected from Western Ghats. It was processed as per microbiological procedure. The observations in terms of morphological, physiological, biochemical and genetical were made for identifications of selected exopolysaccharides producing bacteria. The selected isolate was confirmed as Klebsiella pneumoniae by 16S rRNA sequences. It was taken up for further studies to ascertain the various cultural conditions for exopolysaccharide production. The EPS (exopolysaccharides) producing ability was evaluated by nutritional defined media. The data obtained reveals that the pH, temperature, carbon, nitrogen, aminoacids surfactants, metal ions conditions had the most significant influences. Among the various nutritional sources of interactive effects the maximum EPS production was recorded at pH 7.00 (1.58 ± 0.0096), Temperature at 37℃ , Jaggery (1.37 ± 0.003), Tryptone (1.56 ± 0.012), Ferric chloride (1.98 ± 0.02), Glutamine (2.13 ± 0.067), Polyethylene glycol (0.79 ± 0.043).

KEYWORDS
EPS, Media Optimization, 16S rRNA, Klebsiella pneumoniae

Cite This Paper in IEEE or APA Citation Styles
(a). IEEE Format:
[1] T. Sivakumar , T. Shankar , V. Thangapandian , S. Mahendran , "Media Optimization for Exopolysaccharide Producing Klebsiella pneumoniae KU215681 under Varying Cultural Conditions," International Journal of Biochemistry and Biophysics, Vol. 4, No. 2, pp. 16 - 23, 2016. DOI: 10.13189/ijbb.2016.040202.

(b). APA Format:
T. Sivakumar , T. Shankar , V. Thangapandian , S. Mahendran (2016). Media Optimization for Exopolysaccharide Producing Klebsiella pneumoniae KU215681 under Varying Cultural Conditions. International Journal of Biochemistry and Biophysics, 4(2), 16 - 23. DOI: 10.13189/ijbb.2016.040202.