International Journal of Biochemistry and Biophysics Vol. 1(2), pp. 41 - 45
DOI: 10.13189/ijbb.2013.010203
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CD Spectral Studies of Pb2+ Interference with 5-Fluorouracil Induced DNA Structural Changes


Siva Sankar.K1,*, Veda V Ramineni2, Kaiser Jamil3, Deepak Bhatnagar4
1 Dept of Pediatrics, Health Science Center, State University of New York (SUNY), Stony Brook, New York, USA – 11794
2 Abramson Research Center, Department of Anesthesiology, The Children's Hospital of Philadelphia (CHOP), University of Pennsylvania, Philadelphia, Pennsylvania, USA – 19018
3 Department of Genetics, Bhagwan Mahavir Medical Research Center, Hyderabad, India
4 School of Biochemistry, Devi Ahilya University, Indore, MP, India

ABSTRACT

Lead (Pb2+) a common pollutant and known toxicant, and other various anti cancer drugs such as 5-fluorouracil bind to DNA competitively. Their efficacy as anti cancer drugs was brought by their mechanism of involvement with DNA and their interactive geometries. In this perspective we have designed CD spectral studies to investigate the interference of Pb2+ with DNA-Drug interaction geometries. The change in elliptical intensities at around 260 nm to 280 nm and 245 nm shows the conformational changes in B-form of DNA. Amplitude changes observed with in B-form indicates the hairpin formation which is an intra-molecular variant of the B-form. Calf thymus DNA with 42% of G-C content has tendency towards hairpin transitions. In this study we have found that hairpin transitions might be involved in mechanism of Pb2+ interference with 5-fluorouracil induced structural changes.

KEYWORDS
Lead (Pb2+), 5- Fluorouracil (5FU), Calf Thymus DNA, Circular Dichroism (CD) Spectral Studies

Cite This Paper in IEEE or APA Citation Styles
(a). IEEE Format:
[1] Siva Sankar.K , Veda V Ramineni , Kaiser Jamil , Deepak Bhatnagar , "CD Spectral Studies of Pb2+ Interference with 5-Fluorouracil Induced DNA Structural Changes," International Journal of Biochemistry and Biophysics, Vol. 1, No. 2, pp. 41 - 45, 2013. DOI: 10.13189/ijbb.2013.010203.

(b). APA Format:
Siva Sankar.K , Veda V Ramineni , Kaiser Jamil , Deepak Bhatnagar (2013). CD Spectral Studies of Pb2+ Interference with 5-Fluorouracil Induced DNA Structural Changes. International Journal of Biochemistry and Biophysics, 1(2), 41 - 45. DOI: 10.13189/ijbb.2013.010203.