International Journal of Neuroscience and Behavioral Science Vol. 3(3), pp. 32 - 40
DOI: 10.13189/ijnbs.2015.030302
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Effect of Pimpinella anisum L (Aniseed) Aqueous Extract against Lead (Pb) Neurotoxicity: Neurobehavioral Study


Amina Bekara 1,*, Nadia Ait Hamadouche 1, Khaled Kahloula 2, Sara Harouat 1, Dalila Tabbas 1, Abd-el-Kader Aoues 1
1 Laboratory of Experimental Bio-toxicology, Bio-depollution and Phyto-remediation, Department of Biology, University of Oran 1 (Ahmed Benbella), Algeria
2 Laboratory of Biochemistry, Department of Biology, University of Saida, Algeria

ABSTRACT

Pregnant rats received 0, 2% of lead acetate (Pb) in drinking water. The treatment with the aqueous extract of Pimpinella anisum L (P.A.E.) started after weaning with dose of 750 mg/kg and for 15 successive days. The effect of the extract was evaluated through behavioral tests: open filed (OF) , Forced swimming test ( FST) and dark /light test . Beside; Lactate dehydrogenase (LDH), total plasmatic proteins, alkaline phosphatase (ALP), lipid peroxidation (TBARS) and Catalase (CAT) were analyzed. Lead induced a hyperactivity in the open field which was reduced after administration of P.A.E. In the FST, the treatment with plant extract enhanced significantly the mobility time compared with intoxicated group. We had observed that time spent in the dark compartments in dark and light test was reduced after oral administration of P.A.E. Lead caused significant increase in LDH , TBARS and ALP , in contrary this toxic agent decrease the levels of plasmatic proteins and catalase activity in cerebellum and cerebrum . We can conclude that oral treatment with aniseed aqueous extract was effective in reducing the level of some of biochemical parameters and ameliorate behavior of intoxicated rats by lead.

KEYWORDS
P.anisum L, Aqueous Extract, Lead Acetate, Neurobehavioral Tests, Cerebellum and Cerebrum

Cite This Paper in IEEE or APA Citation Styles
(a). IEEE Format:
[1] Amina Bekara , Nadia Ait Hamadouche , Khaled Kahloula , Sara Harouat , Dalila Tabbas , Abd-el-Kader Aoues , "Effect of Pimpinella anisum L (Aniseed) Aqueous Extract against Lead (Pb) Neurotoxicity: Neurobehavioral Study," International Journal of Neuroscience and Behavioral Science, Vol. 3, No. 3, pp. 32 - 40, 2015. DOI: 10.13189/ijnbs.2015.030302.

(b). APA Format:
Amina Bekara , Nadia Ait Hamadouche , Khaled Kahloula , Sara Harouat , Dalila Tabbas , Abd-el-Kader Aoues (2015). Effect of Pimpinella anisum L (Aniseed) Aqueous Extract against Lead (Pb) Neurotoxicity: Neurobehavioral Study. International Journal of Neuroscience and Behavioral Science, 3(3), 32 - 40. DOI: 10.13189/ijnbs.2015.030302.