International Journal of Biochemistry and Biophysics Vol. 3(1), pp. 1 - 5
DOI: 10.13189/ijbb.2015.030101
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Change in Concentration of Vitamin D2 in Oyster Mushrooms Exposed to 254nm and 365nm UV-light During Growth


Tindibale L. Edward 1,*, M. S. K. Kirui 1, Josiah O. Omolo 2, Richard G. Ngumbu 1, Peter M. Odhiambo 1, Kamweru Paul K. 3
1 Department of Physics, Egerton University, Kenya
2 Department of Chemistry, Egerton University, Kenya
3 Department of Physical Sciences, Chuka University, Kenya

ABSTRACT

The sun emits ultraviolet radiation in form of ultraviolet-A (UV-A), ultraviolet-B (UV-B), and ultraviolet-C (UV-C) bands. Ultraviolet light has the potential to boost vitamin D2 production in mushrooms which human bodies cannot synthesize. The ergosterol in mushrooms, a component of fungal cell membranes which serves the same function as cholesterol in animal cells, can be converted into vitamin D2 by exposure to controlled ultraviolet light. However mushrooms are conventionally grown in the dark, necessitating artificial ultraviolet irradiation. This study investigated the effects of UV-A (365nm) and UV-C (254nm) light exposure time during mushrooms growth, on the concentration of vitamin D2 in oyster mushrooms (Pleurotus ostreatus species) after harvest. Mushrooms samples exposure times were varied from 10-60 minutes per day at intervals of 10 minutes, and irradiation done for three days. UV spectroscopy was used to determine the amounts of Vitamin D2. It was found that the absorbance of vitamin D2 for UV-A light ranged from 0.18-0.49 for the 10-60 minutes of irradiation respectively, while for UV-C light the vitamin D2 content absorbance was 0.38-0.81 for the 10-60 minutes of irradiation respectively. There was a linear relationship between time of irradiation and absorbance vitamin D2 content up to 50 minutes for UV-A and 40 minutes for UV-C.

KEYWORDS
UV-A and UV-C light, Oyster Mushrooms, Vitamin D2, Growth

Cite this paper
Tindibale L. Edward , M. S. K. Kirui , Josiah O. Omolo , Richard G. Ngumbu , Peter M. Odhiambo , Kamweru Paul K. (2015). Change in Concentration of Vitamin D2 in Oyster Mushrooms Exposed to 254nm and 365nm UV-light During Growth. International Journal of Biochemistry and Biophysics, 3 , 1 - 5. doi: 10.13189/ijbb.2015.030101.