International Journal of Biochemistry and Biophysics Vol. 2(3), pp. 15 - 18
DOI: 10.13189/ijbb.2014.020301
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Analysis of Avocado Leaf, Casmir Leaf and Morenga Leaf for the Detection of Concentration of Chlorophyll a and Chlorophyll b


Desissa Yadata *
Mizan Tepi, t office 121, Ethiopia

ABSTRACT

Hotosynthesis can be influenced directly by the concentration of chlorophyll and hence detection of such important pigment is the central point of the work under study. The objective of the work is determination of the concentration of leaf pigments particularly chlorophyll a and chlorophyll b. UV-Visible spectrophotometer is used to determine the absorbance of leaf samples obtained from three different plant species Shiferaw, Casmir and avocado; three replicate samples has been considered in each plant species. The samples collected in each species were preserved at -200℃ and dark area. The sample preserved under such a condition is weighed i.e.; 2g of each fresh leaf sample considered and crashed in 80% acetone and the extract was filtered with what man filter paper No42. After the dilution of the extract the absorbance was determined for chlorophyll a and chlorophyll b at 663nm and 649nm respectively. From the absorbance data; the corresponding concentration determined. According to the calculation concentration of chl a in shiferaw is 0.136mg/Kg in Casmir 0.229mg/Kg and 0.1144 mg/Kg in avocado. The concentration of chlorophyll b in avocado is 0.108mg/kg in shiferaw, 0.214mg/Kg and 0.102 mg/Kg in Casmir leaf sample. The concentration of chlorophyll a is greater than chlorophyll b which coincides with the fact that the abundance of chlorophyll a is greater than the chlorophyll b in most land trees.

KEYWORDS
Vocado, Casmir, Chlorophyll, Leaf Pigments, Photosynthesis, Shiferwa

Cite This Paper in IEEE or APA Citation Styles
(a). IEEE Format:
[1] Desissa Yadata , "Analysis of Avocado Leaf, Casmir Leaf and Morenga Leaf for the Detection of Concentration of Chlorophyll a and Chlorophyll b," International Journal of Biochemistry and Biophysics, Vol. 2, No. 3, pp. 15 - 18, 2014. DOI: 10.13189/ijbb.2014.020301.

(b). APA Format:
Desissa Yadata (2014). Analysis of Avocado Leaf, Casmir Leaf and Morenga Leaf for the Detection of Concentration of Chlorophyll a and Chlorophyll b. International Journal of Biochemistry and Biophysics, 2(3), 15 - 18. DOI: 10.13189/ijbb.2014.020301.