International Journal of Biochemistry and Biophysics Vol. 1(3), pp. 57 - 63
DOI: 10.13189/ijbb.2013.010302
Reprint (PDF) (444Kb)


Pioneer Evaluation of the Possible Side Effects of the DNA Insecticides on Wheat (Triticum Aestivum L.)


Oberemok Volodymyr , Nyadar Palmah *, Zaytsev Oleksii , Levchenko Nataliya , Shiyntum Honore , Omelchenko Olexandr
Department of Biochemistry, Taurida National V.I. Vernadsky University, 95007 Simferopol, Ukraine

ABSTRACT

The paper focuses on the pioneer evaluation of the possible side effects of a novel preparation against phyllophagous insects, DNA insecticides, on some biochemical parameters (concentration of glucose and activity of alkaline phosphatase) of wheat sprouts (Triticum aestivum L.). Our results show that the application of DNA insecticide based on single-stranded DNA fragment from RING domain of IAP-3 gene of gypsy moth multicapsid nuclear polyhedrosis virus results in significant difference in the decreased level of glucose and activity of alkaline phosphatase in wheat sprouts in comparison with the control after treatment on the second day of the experiment. However, the effect becomes significantly unnoticeable after one week when compared with the control. No significant difference in the dried biomass was found between wheat sprouts from the control and the experimental groups after the treatment on the seventh day and after three weeks on the Hoagland’s substrate. Obtained results generally show that agricultural plants are able to overcome the misbalance in the concentration of glucose and activity of alkaline phosphatase caused by the DNA insecticides and emphasize good potential in harmlessness of DNA insecticides that could be used to control phyllophagous insects without substantial side effects on the crop.

KEYWORDS
DNA Insecticides, IAP-3 Gene of LdMNPV, BIR and RING Domains, TnSNPV, ICTVdb, Wheat, Alkaline Phosphatase, Glucose

Cite This Paper in IEEE or APA Citation Styles
(a). IEEE Format:
[1] Oberemok Volodymyr , Nyadar Palmah , Zaytsev Oleksii , Levchenko Nataliya , Shiyntum Honore , Omelchenko Olexandr , "Pioneer Evaluation of the Possible Side Effects of the DNA Insecticides on Wheat (Triticum Aestivum L.)," International Journal of Biochemistry and Biophysics, Vol. 1, No. 3, pp. 57 - 63, 2013. DOI: 10.13189/ijbb.2013.010302.

(b). APA Format:
Oberemok Volodymyr , Nyadar Palmah , Zaytsev Oleksii , Levchenko Nataliya , Shiyntum Honore , Omelchenko Olexandr (2013). Pioneer Evaluation of the Possible Side Effects of the DNA Insecticides on Wheat (Triticum Aestivum L.). International Journal of Biochemistry and Biophysics, 1(3), 57 - 63. DOI: 10.13189/ijbb.2013.010302.